Microbial Biotechnology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India.
BMC Complement Med Ther. 2020 Nov 5;20(1):331. doi: 10.1186/s12906-020-03112-1.
Due to concerns regarding the safety of the chemical control measures, the trend is shifting globally towards the use of natural compounds as antimicrobial agent especially, plant essential oils.
This study presented the antibacterial potential of Cymbopogon khasianus essential oil (CKEO) against human pathogens: Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis, Staphylococcus aureus and Candida albicans with MIC ranging from 20 to 100 μg/mL. CKEO, in comparison to its major constituent, geraniol, showed better MICs against tested pathogens. In combination studies, the effective concentrations of CKEO and streptomycin were reduced from 20 to 5 μg/mL and 11 to 0.7 ng/mL against E. coli. This suggests their synergistic action. However, CKEO showed partial synergy with ciprofloxacin. To understand the efficacy of CKEO, time-kill kinetics was performed. CKEO took the half time to show the bactericidal effect in comparison to streptomycin at their 2x MICs (double the MIC), while their combination took only 30 min for this. Fluorescence and surface electron microscopic and protein estimation studies suggested the multi-target action of CKEO-streptomycin combination against E. coli. Further, CKEO alone/in combination exhibited less than 10% haemolytic activity at its MIC.
These results indicate that CKEO is a potentially safe alternative for the treatment of various pathogenic bacterial strains. It could be used for a variety of applications including human health, food storage, aquaculture, etc.
由于对化学控制措施安全性的担忧，全球范围内的趋势正在转向使用天然化合物作为抗菌剂，尤其是植物精油。
本研究展示了香茅精油（CKEO）对人类病原体的抗菌潜力：铜绿假单胞菌、鼠伤寒沙门氏菌、大肠杆菌、肺炎克雷伯菌、枯草芽孢杆菌、金黄色葡萄球菌和白色念珠菌，其 MIC 范围为 20 至 100μg/mL。与主要成分香叶醇相比，CKEO 对测试病原体的 MIC 更好。在联合研究中，CKEO 和链霉素的有效浓度从 20 降至 5μg/mL 和 11 降至 0.7ng/mL，对大肠杆菌有效。这表明它们具有协同作用。然而，CKEO 与环丙沙星表现出部分协同作用。为了了解 CKEO 的疗效，进行了时间杀伤动力学研究。与链霉素在其 2x MIC（MIC 的两倍）相比，CKEO 达到杀菌效果的半衰期更短，而它们的组合仅需 30 分钟。荧光和表面电子显微镜以及蛋白质估计研究表明，CKEO-链霉素联合对大肠杆菌具有多靶点作用。此外，CKEO 单独/联合使用时，在其 MIC 下的溶血活性低于 10%。
这些结果表明，CKEO 是治疗各种病原菌的潜在安全替代品。它可用于多种应用，包括人类健康、食品储存、水产养殖等。
