Svitina Hanna, Kyryk Vitaliy, Skrypkina Inessa, Kuchma Maria, Bukreieva Tetiana, Areshkov Pavlo, Shablii Yulia, Denis Yevheniy, Klymenko Pavlo, Garmanchuk Liudmyla, Ostapchenko Liudmyla, Lobintseva Galina, Shablii Volodymyr
Cell Culture Laboratory, Cryobank, Institute of Cell Therapy, 03680 Kyiv, Ukraine.
Department of Biochemistry, Educational and Scientific Centre "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine.
Exp Ther Med. 2017 Sep;14(3):2135-2147. doi: 10.3892/etm.2017.4792. Epub 2017 Jul 12.
Transplantation of placenta-derived multipotent cells (PDMCs) is a promising approach for cell therapy to treat inflammation-associated colon diseases. However, the effect of PDMCs on colon cancer cells remains unknown. The aim of the present study was to characterize PDMCs obtained from human (hPDMCs) and rat (rPDMCs) placentas and to evaluate their impact on colon cancer progression in rats. PDMCs were obtained from human and rat placentas by tissue explant culturing. Stemness- and trophoblast-related gene expression was studied using reverse transcription-polymerase chain reaction (RT-PCR), and surface markers and intracellular proteins were detected using flow cytometry and immunofluorescence, respectively. Experimental colon carcinogenesis was induced in male albino Wistar rats by injecting 20 mg/kg dimethylhydrazine (DMH) once a week for 20 consecutive weeks. The administration of rPDMCs and hPDMC was performed at week 22 after the initial DMH-injection. All animals were sacrificed through carbon dioxide asphyxiation at week 5 after cell transplantation. The number and size of each tumor lesion was calculated. The type of tumor was determined by standard histological methods. Cell engraftment was determined by PCR and immunofluorescence. Results demonstrated that rPDMCs possessed the immunophenotype and differentiation potential inherent in MSCs; however, hPDMCs exhibited a lower expression of cluster of differentiation 44 and did not express trophoblast-associated genes. The data of the present study indicated that PDMCs may engraft in different tissues but do not significantly affect DMH-induced tumor growth during short-term observations.
胎盘来源的多能细胞（PDMCs）移植是一种用于细胞治疗以治疗炎症相关结肠疾病的有前景的方法。然而，PDMCs对结肠癌细胞的影响仍不清楚。本研究的目的是鉴定从人（hPDMCs）和大鼠（rPDMCs）胎盘中获得的PDMCs，并评估它们对大鼠结肠癌进展的影响。通过组织外植体培养从人胎盘和大鼠胎盘中获得PDMCs。使用逆转录-聚合酶链反应（RT-PCR）研究干性和滋养层相关基因的表达，分别使用流式细胞术和免疫荧光检测表面标志物和细胞内蛋白。通过每周一次注射20mg/kg二甲基肼（DMH），连续20周，诱导雄性白化Wistar大鼠发生实验性结肠癌。在首次注射DMH后的第22周进行rPDMCs和hPDMC的给药。在细胞移植后的第5周，通过二氧化碳窒息处死所有动物。计算每个肿瘤病变的数量和大小。通过标准组织学方法确定肿瘤类型。通过PCR和免疫荧光确定细胞植入情况。结果表明，rPDMCs具有间充质干细胞固有的免疫表型和分化潜能；然而，hPDMCs的分化簇44表达较低，且不表达滋养层相关基因。本研究数据表明，PDMCs可能植入不同组织，但在短期观察期间对DMH诱导的肿瘤生长没有显著影响。
