• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

[具体物质]对大鼠糖皮质激素性骨质疏松的预防作用

Preventive effects of on glucocorticoid-induced osteoporosis in rats.

作者信息

Zhou Manru, Li Jin, Wu Jingkai, Yang Yajun, Zeng Xiaobing, Lv Xiaohua, Cui Liao, Yao Weimin, Liu Yuyu

机构信息

Department of Pharmacology, Guangdong Medical University, Zhanjiang, Guangdong 524023, P.R. China.

Department of Pharmacy, Xinhua College of Sun Yat-sen University, Guangzhou, Guangdong 510520, P.R. China.

出版信息

Exp Ther Med. 2017 Sep;14(3):2445-2460. doi: 10.3892/etm.2017.4802. Epub 2017 Jul 18.

DOI:10.3892/etm.2017.4802
PMID:28962180
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5609219/
Abstract

In Traditional Chinese Medicine, (PM) is known for its anti-aging properties. A previous study by our group showed that extracts of PM were able to prevent and treat bone loss , and the active components emodin and 2,3,5,4,-tetrahydroxystilbene-2--β-glucoside (TSG) promoted the osteogenic differentiation of mesenchymal stem cells . The aim of the present study was to investigate the preventive effects of PM on glucocorticoid-induced osteoporosis (GIO) in rats. A crude extract of PM was prepared with 75% ethanol, purified and enriched using a D-101 macroresin column and elution with 30% ethanol, and the material obtained was assessed by high-performance liquid chromatography. Male or female Sprague Dawley rats (n=180) were randomly divided into nine groups: Control, prednisone, prednisone plus calcitriol (CAL), prednisone plus 30% ethanolic eluate of PM [high (H), medium (M) and low (L) dose] and prednisone plus crude extract of PM (H, M and L dose). Prednisone was orally administered to the osteoporosis model rats for 21 weeks, alongside which they received PM extracts. The weight of the viscera, anterior tibial muscle and other tissues was recorded at the end of the experiment. The femur and lumbar vertebra were collected for the measurement of three-dimensional microarchitecture by micro-computed tomography scanning, assessment of biomechanical properties and determination of bone mineral density (BMD). In the 30% ethanolic eluate of the PM extract, the content of TSG and combined anthraquinone was 9.20 and 0.15%, respectively, and that in the crude extract of PM was 2.23 and 0.03%, respectively. Over 6 weeks, the weight of the rats the in prednisone group decreased (P<0.05), while the weight of rats treated with M and H doses of 30% ethanolic eluate was increased compared with that in the prednisone group (P<0.05). Rats exposed to prednisone exhibited a deteriorated bone microarchitecture, low BMD, decreased bone volume/total volume and poor biomechanical properties. Furthermore, the weight of the adrenal gland and the anterior tibial muscle was decreased. 30% ethanolic eluate of PM at M and L doses and crude extract of PM at the H dose counteracted the alterations of skeletal and other characteristics induced by prednisone in rats, as did CAL. In conclusion, extracts of PM exerted a protective effect on bone tissue in GIO rats.

摘要

在传统中医中，（此处原文“(PM)”指代不明，可能有信息缺失）以其抗衰老特性而闻名。我们团队之前的一项研究表明，该提取物能够预防和治疗骨质流失，其活性成分大黄素和2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷（TSG）可促进间充质干细胞的成骨分化。本研究的目的是探讨该提取物对大鼠糖皮质激素诱导性骨质疏松症（GIO）的预防作用。用75%乙醇制备该提取物的粗提物，通过D - 101大孔树脂柱进行纯化和富集，并用30%乙醇洗脱，所得物质通过高效液相色谱进行评估。将雄性或雌性斯普拉格 - 道利大鼠（n = 180）随机分为九组：对照组、泼尼松组、泼尼松加骨化三醇（CAL）组、泼尼松加提取物30%乙醇洗脱液[高（H）、中（M）和低（L）剂量]组以及泼尼松加提取物粗提物（H、M和L剂量）组。对骨质疏松模型大鼠口服泼尼松21周，同时给予提取物。实验结束时记录内脏、胫前肌和其他组织的重量。收集股骨和腰椎，通过微计算机断层扫描测量三维微观结构，评估生物力学性能并测定骨密度（BMD）。提取物30%乙醇洗脱液中TSG和总蒽醌的含量分别为9.20%和0.15%，提取物粗提物中分别为2.23%和0.03%。在6周内，泼尼松组大鼠体重下降（P < 0.05），而给予M和H剂量30%乙醇洗脱液治疗的大鼠体重比泼尼松组增加（P < 0.05）。接受泼尼松的大鼠表现出骨微观结构恶化、骨密度低、骨体积/总体积降低和生物力学性能差。此外，肾上腺和胫前肌的重量减少。M和L剂量的提取物30%乙醇洗脱液以及H剂量的提取物粗提物可抵消泼尼松诱导的大鼠骨骼和其他特征的改变，CAL组也是如此。总之，提取物对GIO大鼠的骨组织具有保护作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/dccb37f58fea/etm-14-03-2445-g11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/85d93ed68b54/etm-14-03-2445-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/3da15d926fd6/etm-14-03-2445-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/d331436a5f75/etm-14-03-2445-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/b3ff3b352143/etm-14-03-2445-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/f6d0ef10b590/etm-14-03-2445-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/cda96579da27/etm-14-03-2445-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/8f123e5cb816/etm-14-03-2445-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/19d4dbc3a640/etm-14-03-2445-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/3134f687624d/etm-14-03-2445-g09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/7023b56eea07/etm-14-03-2445-g10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/dccb37f58fea/etm-14-03-2445-g11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/85d93ed68b54/etm-14-03-2445-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/3da15d926fd6/etm-14-03-2445-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/d331436a5f75/etm-14-03-2445-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/b3ff3b352143/etm-14-03-2445-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/f6d0ef10b590/etm-14-03-2445-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/cda96579da27/etm-14-03-2445-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/8f123e5cb816/etm-14-03-2445-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/19d4dbc3a640/etm-14-03-2445-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/3134f687624d/etm-14-03-2445-g09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/7023b56eea07/etm-14-03-2445-g10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c60e/5609219/dccb37f58fea/etm-14-03-2445-g11.jpg

相似文献

1
Preventive effects of on glucocorticoid-induced osteoporosis in rats.[具体物质]对大鼠糖皮质激素性骨质疏松的预防作用
Exp Ther Med. 2017 Sep;14(3):2445-2460. doi: 10.3892/etm.2017.4802. Epub 2017 Jul 18.
2
Polygonum multiflorm alleviates glucocorticoid‑induced osteoporosis and Wnt signaling pathway.多花蓼缓解糖皮质激素诱导的骨质疏松症及 Wnt 信号通路。
Mol Med Rep. 2018 Jan;17(1):970-978. doi: 10.3892/mmr.2017.7997. Epub 2017 Nov 7.
3
Tetrahydroxystilbene glucoside isolated from Thunb. demonstrates osteoblast differentiation promoting activity.从何首乌中分离得到的四羟基二苯乙烯苷具有促进成骨细胞分化的活性。
Exp Ther Med. 2017 Oct;14(4):2845-2852. doi: 10.3892/etm.2017.4915. Epub 2017 Aug 9.
4
Investigation of the idiosyncratic hepatotoxicity of Polygonum multiflorum Thunb. through metabolomics using GC-MS.采用 GC-MS 代谢组学方法研究何首乌的特异肝毒性。
BMC Complement Med Ther. 2021 Apr 12;21(1):120. doi: 10.1186/s12906-021-03276-4.
5
Polygonum multiflorum Thunb. Induces hepatotoxicity in SD rats and hepatocyte spheroids by Disrupting the metabolism of bilirubin and bile acid.何首乌通过破坏胆红素和胆汁酸的代谢在 SD 大鼠和肝细胞球中诱导肝毒性。
J Ethnopharmacol. 2022 Oct 5;296:115461. doi: 10.1016/j.jep.2022.115461. Epub 2022 Jun 18.
6
Pharmacokinetics and tissue distribution of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside from traditional Chinese medicine Polygonum multiflorum following oral administration to rats.中药何首乌中二苯乙烯苷在大鼠体内的药代动力学和组织分布。
J Ethnopharmacol. 2011 Sep 1;137(1):449-56. doi: 10.1016/j.jep.2011.05.049. Epub 2011 Jun 6.
7
Comparative Studies on Multi-Component Pharmacokinetics of Thunb Extract After Oral Administration in Different Rat Models.不同大鼠模型口服独活提取物后多组分药代动力学的比较研究
Front Pharmacol. 2021 Jun 17;12:655332. doi: 10.3389/fphar.2021.655332. eCollection 2021.
8
Extract and fraction of Cassia occidentalis L. (a synonym of Senna occidentalis) have osteogenic effect and prevent glucocorticoid-induced osteopenia.番泻叶（其同义词为西方山扁豆）的提取和分离物具有成骨作用，可预防糖皮质激素引起的骨质疏松症。
J Ethnopharmacol. 2019 May 10;235:8-18. doi: 10.1016/j.jep.2019.01.029. Epub 2019 Jan 28.
9
Protective Effects of 2,3,5,4'-Tetrahydroxystilbene-2--β-d-glucoside on Ovariectomy Induced Osteoporosis Mouse Model.2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷对去卵巢诱导骨质疏松症小鼠模型的保护作用。
Int J Mol Sci. 2018 Aug 28;19(9):2554. doi: 10.3390/ijms19092554.
10
Hepatotoxic assessment of Polygoni Multiflori Radix extract and toxicokinetic study of stilbene glucoside and anthraquinones in rats.何首乌提取物的肝毒性评估及大鼠中二苯乙烯苷和蒽醌的毒代动力学研究
J Ethnopharmacol. 2015 Mar 13;162:61-8. doi: 10.1016/j.jep.2014.12.045. Epub 2014 Dec 31.

引用本文的文献

1
Systematic Myostatin Expression Screening Platform for Identification and Evaluation of Myogenesis-Related Phytogenic in Pigs.用于鉴定和评估猪肌生成相关植物源物质的系统性肌肉生长抑制素表达筛选平台
Bioengineering (Basel). 2023 Sep 22;10(10):1113. doi: 10.3390/bioengineering10101113.
2
Animal Model for Glucocorticoid Induced Osteoporosis: A Systematic Review from 2011 to 2021.糖皮质激素性骨质疏松动物模型的系统评价：2011 年至 2021 年的研究。
Int J Mol Sci. 2021 Dec 29;23(1):377. doi: 10.3390/ijms23010377.
3
Allicin Reversed the Process of Frailty in Aging Male Fischer 344 Rats With Osteoporosis.

本文引用的文献

1
Anti-osteoporotic activity of harpagide by regulation of bone formation in osteoblast cell culture and ovariectomy-induced bone loss mouse models.通过调节成骨细胞培养中的骨形成以及卵巢切除诱导的骨质流失小鼠模型，哈帕苷的抗骨质疏松活性。
J Ethnopharmacol. 2016 Feb 17;179:66-75. doi: 10.1016/j.jep.2015.12.025. Epub 2015 Dec 19.
2
Molecular Biology of Glucocorticoid Signaling.糖皮质激素信号传导的分子生物学
Adv Exp Med Biol. 2015;872:33-57. doi: 10.1007/978-1-4939-2895-8_2.
3
Secondary osteoporosis in collagen-induced arthritis rats.胶原诱导性关节炎大鼠的继发性骨质疏松症
大蒜素逆转骨质疏松症雄性 Fischer 344 衰老大鼠虚弱进程。
J Gerontol A Biol Sci Med Sci. 2020 Apr 17;75(5):821-825. doi: 10.1093/gerona/glz205.
4
Polygonum multiflorm alleviates glucocorticoid‑induced osteoporosis and Wnt signaling pathway.多花蓼缓解糖皮质激素诱导的骨质疏松症及 Wnt 信号通路。
Mol Med Rep. 2018 Jan;17(1):970-978. doi: 10.3892/mmr.2017.7997. Epub 2017 Nov 7.
J Bone Miner Metab. 2016 Sep;34(5):500-16. doi: 10.1007/s00774-015-0700-4. Epub 2015 Jul 26.
4
Analysis of quality improvement efforts in preventing glucocorticoid-induced osteoporosis.分析预防糖皮质激素性骨质疏松症的质量改进措施。
Semin Arthritis Rheum. 2015 Apr;44(5):483-488. doi: 10.1016/j.semarthrit.2014.09.011.
5
Genistein attenuates glucocorticoid-induced bone deleterious effects through regulation Eph/ephrin expression in aged mice.金雀异黄素通过调节老年小鼠中Eph/ephrin的表达减轻糖皮质激素诱导的骨骼有害影响。
Int J Clin Exp Pathol. 2015 Jan 1;8(1):394-403. eCollection 2015.
6
Application of microscopy technique and high performance liquid chromatography for quality assessment of Polygonum multiflorum Thunb. (Heshouwu).显微镜技术和高效液相色谱法在何首乌质量评价中的应用。
Pharmacogn Mag. 2014 Oct;10(40):415-21. doi: 10.4103/0973-1296.141788.
7
Glucocorticoid-induced osteoporosis: 2013 update.糖皮质激素性骨质疏松症：2013年更新版
Reumatismo. 2014 Jul 28;66(2):144-52. doi: 10.4081/reumatismo.2014.787.
8
Acute murine antigen-induced arthritis is not affected by disruption of osteoblastic glucocorticoid signalling.急性鼠源抗原诱导性关节炎不受成骨细胞糖皮质激素信号转导破坏的影响。
BMC Musculoskelet Disord. 2014 Feb 3;15:31. doi: 10.1186/1471-2474-15-31.
9
Advanced oxidation protein products accelerate bone deterioration in aged rats.高级氧化蛋白产物加速老年大鼠的骨骼恶化。
Exp Gerontol. 2014 Feb;50:64-71. doi: 10.1016/j.exger.2013.11.014. Epub 2013 Dec 4.
10
A novel flavonoid C-glucoside from Ulmus wallichiana preserves bone mineral density, microarchitecture and biomechanical properties in the presence of glucocorticoid by promoting osteoblast survival: a comparative study with human parathyroid hormone.从榆属植物中分离得到的一种新型黄酮 C-糖苷通过促进成骨细胞存活来维持糖皮质激素存在下的骨密度、微结构和生物力学特性：与人甲状旁腺激素的比较研究。
Phytomedicine. 2013 Nov 15;20(14):1256-66. doi: 10.1016/j.phymed.2013.07.007. Epub 2013 Aug 6.