Vedi Mahima, Rasool Mahaboobkhan, Sabina Evan Prince
VIT University, Vellore 632014 Tamil Nadu, India.
Toxicol Rep. 2014 Aug 27;1:629-638. doi: 10.1016/j.toxrep.2014.08.009. eCollection 2014.
The present study investigated the possible protective role of (Linn.) Dunal (Solanaceae) root powder against bromobenzene-induced oxidative damage in rat liver mitochondria. Administration of bromobenzene (10 mmol/kg body weight) to rats resulted in increased levels of liver marker enzymes, lipid peroxidation, TNF-α, IL-1β and VEGF. There was also marked depletion in the levels of mitochondrial enzymes and antioxidant activity. Pre-treatment with significantly decreased the levels of liver marker enzymes, TNF-α, IL-1β, VEGF and ameliorated histopathological manifestations in bromobenzene-treated rats. The molecular docking analysis predicted that the pro-inflammatory mediator NF-κB showed significant interaction with selected various active components of (withaferin A, withanolide D and withanolide E). This study demonstrates a good protective effect of against bromobenzene-induced oxidative stress.
本研究调查了刺蒺藜（茄科）根粉对溴苯诱导的大鼠肝线粒体氧化损伤可能的保护作用。给大鼠施用溴苯（10 mmol/kg体重）导致肝脏标志物酶水平、脂质过氧化、肿瘤坏死因子-α、白细胞介素-1β和血管内皮生长因子升高。线粒体酶水平和抗氧化活性也显著降低。用刺蒺藜根粉预处理可显著降低溴苯处理大鼠的肝脏标志物酶、肿瘤坏死因子-α、白细胞介素-1β、血管内皮生长因子水平，并改善组织病理学表现。分子对接分析预测，促炎介质核因子-κB与刺蒺藜选定的各种活性成分（白屈菜红碱A、睡茄内酯D和睡茄内酯E）有显著相互作用。本研究证明刺蒺藜对溴苯诱导的氧化应激具有良好的保护作用。
