印度獐牙菜对溴苯诱发的大鼠肝线粒体氧化应激的有益作用。
Beneficial effect of the administration of Hemidesmus indicus against bromobenzene induced oxidative stress in rat liver mitochondria.
机构信息
Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India.
出版信息
J Ethnopharmacol. 2010 Jan 8;127(1):200-3. doi: 10.1016/j.jep.2009.09.043. Epub 2009 Sep 30.
AIM OF THE STUDY
To study the beneficial effect of the prior administration of an aqueous extract of Hemidesmus indicus against bromobenzene induced oxidative damage in rat liver mitochondria.
MATERIALS AND METHODS
Oxidative stress was induced in rats with bromobenzene (10 mmol/kg body wt.). The rate of respiration, P/O ratios, lipid peroxides, protein carbonyls and sulphydryls were studied.
RESULTS
When the rats were administered with bromobenzene, the rate of respiration was decreased significantly and the P/O ratio was completely abolished. There was a significant increase on the levels of lipid peroxide and protein carbonyl and a significant decrease on total sulphydryl groups when compared with control. Administration of rats with an aqueous extract (100mg/kg) prior to bromobenzene administration showed significant beneficial effects like, stimulation in respiration, prevented the rise in lipid peroxides and protein carbonyls, increased the level of sulphydryl groups back to control level. Administration of vitamin E could not reverse as effectively as Hemidesmus indicus.
CONCLUSIONS
This study demonstrates a good protective effect of Hemidesmus indicus against the bromobenzene induced oxidative stress.
研究目的
研究印度獐牙菜水提物预先给药对溴苯诱发的大鼠肝线粒体氧化损伤的有益作用。
材料与方法
用溴苯（10mmol/kg 体重）诱导大鼠产生氧化应激。研究了呼吸速率、P/O 比、脂质过氧化物、蛋白羰基和巯基。
结果
当大鼠给予溴苯时，呼吸速率显著下降，P/O 比完全消失。与对照组相比，脂质过氧化物和蛋白羰基的水平显著增加，总巯基的水平显著下降。在给予溴苯之前给予大鼠水提物（100mg/kg），表现出显著的有益作用，如刺激呼吸、防止脂质过氧化物和蛋白羰基升高、将巯基水平恢复到对照水平。给予维生素 E 不能像印度獐牙菜那样有效地逆转。
结论
本研究表明，印度獐牙菜对溴苯诱导的氧化应激具有良好的保护作用。
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