Pauksch Linda, Rohnke Marcus, Schnettler Reinhard, Lips Katrin S
Laboratory for Experimental Trauma Surgery, Justus-Liebig University Giessen, Schubertstrasse 81, 35392 Giessen, Germany.
Institute for Physical Chemistry, Justus-Liebig University Giessen, Heinrich-Buff-Ring 58, 35392 Giessen, Germany.
Toxicol Rep. 2014 Oct 18;1:900-908. doi: 10.1016/j.toxrep.2014.10.012. eCollection 2014.
Based on the increasing number of multi-drug resistant bacteria in periprosthetic infections, improvement of the antibacterial activity of commonly used biomaterials must be achieved. The broad-spectrum, high antimicrobial efficacy has made silver nanoparticles a promising new antibacterial agent. However, there is still a serious lack of knowledge concerning the impact of nanosilver on bone cells. For this reason a study was conducted to evaluate the influence of silver nanoparticles on osteoclastogenesis of human peripheral blood mononuclear cells. Upon incubation with subtoxic concentrations of nanosilver the cells did not exhibit changes in osteoclast differentiation and podosomal structures. However, the osteoclasts were able to uptake the nanoparticles, accumulating them in endo-lysosomal compartments. Furthermore, nanosilver exposure led to an increase in oxidative stress and a decrease in clathrin-dependent endocytosis on the mRNA level. In conclusion, our results indicate nanosilver-induced cell stress at higher concentrations. For this reason antibacterial benefits and possible health risks should be weighed in more detail in further studies.
基于假体周围感染中多重耐药菌数量的增加，必须提高常用生物材料的抗菌活性。银纳米颗粒具有广谱、高效的抗菌效果，成为一种很有前景的新型抗菌剂。然而，关于纳米银对骨细胞影响的知识仍然严重匮乏。因此，开展了一项研究来评估银纳米颗粒对人外周血单核细胞破骨细胞生成的影响。用亚毒性浓度的纳米银孵育后，细胞在破骨细胞分化和足体结构方面未表现出变化。然而，破骨细胞能够摄取纳米颗粒，并将它们积聚在内溶酶体区室中。此外，纳米银暴露导致氧化应激增加，且在mRNA水平上网格蛋白依赖性内吞作用减少。总之，我们的结果表明高浓度的纳米银会诱导细胞应激。因此，在进一步研究中应更详细地权衡抗菌益处和可能的健康风险。
