Pokrowiecki Rafał, Zaręba Tomasz, Szaraniec Barbara, Pałka Krzysztof, Mielczarek Agnieszka, Menaszek Elżbieta, Tyski Stefan
Center for Cranio-Maxillo-Facial Surgery, Voivodeship Children's Hospital, Olsztyn.
Department of Oral Surgery, Jagiellonian Medical University, Kraków.
Int J Nanomedicine. 2017 Jun 6;12:4285-4297. doi: 10.2147/IJN.S131163. eCollection 2017.
The addition of an antibacterial agent to dental implants may provide the opportunity to decrease the percentage of implant failures due to peri-implantitis. For this purpose, in this study, the potential efficacy of nanosilver-doped titanium biomaterials was determined. Titanium disks were incorporated with silver nanoparticles over different time periods by Tollens reaction, which is considered to be an eco-friendly, cheap, and easy-to-perform method. The surface roughness, wettability, and silver release profile of each disc were measured. In addition, the antibacterial activity was also evaluated by using disk diffusion tests for bacteria frequently isolated from the peri-implant biofilm: , and . Cytotoxicity was evaluated in vitro in a natural human osteoblasts cell culture. The addition of nanosilver significantly increased the surface roughness and decreased the wettability in a dose-dependent manner. These surfaces were significantly toxic to all the tested bacteria following a 48-hour exposure, regardless of silver doping duration. A concentration of 0.05 ppm was sufficient to inhibit Gram-positive and Gram-negative species, with the latter being significantly more susceptible to silver ions. However, after the exposure of human osteoblasts to 0.1 ppm of silver ions, a significant decrease in cell viability was observed by using ToxiLight™ BioAssay Kit after 72 hours. Data from the present study indicated that the incorporation of nanosilver may influence the surface properties that are important in the implant healing process. The presence of nanosilver on the titanium provides an antibacterial activity related to the bacteria involved in peri-implantitis. Finally, the potential toxicological considerations of nanosilver should further be investigated, as both the antibacterial and cytotoxic properties may be observed at similar concentration ranges.
在牙科植入物中添加抗菌剂可能为降低因种植体周围炎导致的植入失败率提供机会。为此，在本研究中，测定了纳米银掺杂钛生物材料的潜在功效。通过托伦斯反应在不同时间段将银纳米颗粒掺入钛盘，该反应被认为是一种环保、廉价且易于操作的方法。测量了每个圆盘的表面粗糙度、润湿性和银释放曲线。此外，还通过对种植体周围生物膜中常见分离细菌进行纸片扩散试验来评估抗菌活性： 、 和 。在天然人成骨细胞培养物中进行体外细胞毒性评估。纳米银的添加显著增加了表面粗糙度，并以剂量依赖方式降低了润湿性。无论银掺杂持续时间如何，在暴露48小时后，这些表面对所有测试细菌均具有显著毒性。0.05 ppm的浓度足以抑制革兰氏阳性菌和革兰氏阴性菌，其中革兰氏阴性菌对银离子更敏感。然而，在人成骨细胞暴露于0.1 ppm银离子后，使用ToxiLight™生物检测试剂盒在72小时后观察到细胞活力显著下降。本研究数据表明，纳米银的掺入可能会影响植入物愈合过程中重要的表面特性。钛表面纳米银的存在提供了与种植体周围炎相关细菌有关的抗菌活性。最后，由于在相似浓度范围内可能同时观察到抗菌和细胞毒性特性，纳米银潜在的毒理学问题应进一步研究。
