Rahmani Helia, Moloudi Mohammad Raman, Hashemi Paria, Hassanzadeh Kambiz, Izadpanah Esmael
Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Physiology and Pharmacology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Neurochem Res. 2023 Jun;48(6):1775-1782. doi: 10.1007/s11064-023-03860-9. Epub 2023 Jan 23.
Huntington's disease (HD) is a progressive, neurodegenerative, and inherited disease. Antioxidants have been shown to be effective in slowing disease progression in animal models of HD and are under investigation in human clinical trials. α-pinene, a member of the monoterpene class, has been shown to exert antioxidant activity. Therefore, this study aimed to investigate the impact of α-pinene on animal model of HD. Thirty-two male Wistar rats received 3-Nitropropionic acid (3-NP) for induction of the disease model or treated with α-pinene + 3-NP in different groups. Motor skill, and biochemical evaluations to detect oxidant/antioxidant markers in rat cortex and striatum were performed in all groups. We found that α-pinene significantly improved 3-NP-induced changes in the body weight, rotarod activity, time taken to cross the narrow beam, and locomotor activity. Biochemical analysis revealed that α-pinene significantly decreased the 3NP-induced elevation in oxidant markers, nitrite, and malondialdehyde in both cortex and striatum. In addition, α-pinene counteracted the 3-NP-induced fall in antioxidant enzymes, including superoxide dismutase, catalase, and glutathione in the cortex and striatum. In conclusion, we found that α-pinene prevented the motor dysfunction induced by 3-NP in the animal model of Huntington's disease. Oxidants-antioxidant balance might be involved in the protective effect of α-pinene.
亨廷顿病（HD）是一种进行性、神经退行性遗传病。抗氧化剂已被证明在HD动物模型中可有效减缓疾病进展，目前正在进行人体临床试验。α-蒎烯是单萜类的一种，已被证明具有抗氧化活性。因此，本研究旨在探讨α-蒎烯对HD动物模型的影响。32只雄性Wistar大鼠分别接受3-硝基丙酸（3-NP）诱导疾病模型或在不同组中用α-蒎烯+3-NP进行治疗。对所有组进行运动技能以及检测大鼠皮质和纹状体中氧化/抗氧化标志物的生化评估。我们发现α-蒎烯显著改善了3-NP诱导的体重、转棒活动、穿过窄梁所需时间和运动活动的变化。生化分析显示，α-蒎烯显著降低了3-NP诱导的皮质和纹状体中氧化标志物、亚硝酸盐和丙二醛的升高。此外，α-蒎烯抵消了3-NP诱导的皮质和纹状体中抗氧化酶（包括超氧化物歧化酶、过氧化氢酶和谷胱甘肽）的下降。总之，我们发现α-蒎烯可预防亨廷顿病动物模型中由3-NP诱导的运动功能障碍。氧化-抗氧化平衡可能参与了α-蒎烯的保护作用。
