Biochemistry Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung, Indonesia.
Appl Biochem Biotechnol. 2012 Nov;168(5):1065-75. doi: 10.1007/s12010-012-9841-2. Epub 2012 Aug 25.
The potential of the tropical marine benthic-diatom Navicula sp. for biodiesel feedstock was investigated. Growth profiles were analyzed by changing nutrient compositions in three different media (Walne, plain seawater, and modified seawater) and irradiance intensities. Navicula sp. cells showed significant growth in Walne and modified seawater medium but not in plain seawater medium. The microalgae grew well in a pH range of 7.8-8.4, and the cells were very sensitive to the intensity of direct sunlight exposure. The average cell concentration obtained from the cultures in plain seawater, Walne, and modified seawater media at the beginning of the stationary phase was 0.70, 2.17, and 2.54 g/L, respectively. Electron spray ionization-ion trap-mass spectrometry showed that the triacylglycerols of the algae oil were identified as POP (palmitic-oleic-palmitic), POO (palmitic-oleic-oleic), and OOLn (oleic-oleic-linoleic). The oil productivity of Navicula sp. cultivated in Walne and modified seawater media was 90 and 124 μL L(-1) culture d(-1). The Navicula sp. biodiesel exhibited a kinematic viscosity of 1.299 mm(2)/s, density of 0.8347 g/mL, and internal energy of 0.90 kJ/mL.
对热带海洋底栖硅藻 Navicula sp. 作为生物柴油原料的潜力进行了研究。通过在三种不同的培养基（Walne、普通海水和改良海水）和光照强度下改变营养成分来分析生长曲线。Navicula sp. 在 Walne 和改良海水中的细胞表现出明显的生长，但在普通海水中的细胞没有生长。微藻在 pH 值为 7.8-8.4 的范围内生长良好，对阳光直射强度非常敏感。在静止期初期，从普通海水、Walne 和改良海水培养基中培养的细胞的平均细胞浓度分别为 0.70、2.17 和 2.54 g/L。电喷雾电离-离子阱-质谱分析表明，藻类油的三酰基甘油被鉴定为 POP（棕榈酸-油酸-棕榈酸）、POO（棕榈酸-油酸-油酸）和 OOLn（油酸-油酸-亚油酸）。在 Walne 和改良海水中培养的 Navicula sp. 的产油率分别为 90 和 124 μL/L·d。Navicula sp. 生物柴油的运动粘度为 1.299 mm(2)/s，密度为 0.8347 g/mL，内能为 0.90 kJ/mL。
