Feronato Cesar, Leme Raquel A, Diniz Jaqueline A, Agnol Alais Maria Dall, Alfieri Alice F, Alfieri Amauri A
Laboratory of Animal Virology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, Paraná, CEP 86057-970, Brazil.
Multi-User Animal Health Laboratory - Molecular Biology Unit, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid - Campus Universitário, PO Box 10011, Londrina, Paraná, CEP 86057-970, Brazil.
Trop Anim Health Prod. 2018 Feb;50(2):337-344. doi: 10.1007/s11250-017-1436-z. Epub 2017 Sep 29.
Senecavirus A (SVA) has been associated with vesicular disease in weaned and adult pigs and with high mortality of newborn piglets. This study aimed to establish a nested-PCR assay for the routine diagnosis of SVA infection. Tissue samples (n = 177) were collected from 37 piglets of 18 pig farms located in four different Brazilian states. For the nested-PCR, a primer set was defined to amplify an internal VP1 fragment of 316 bp of SVA genome. Of the 37 piglets, 15 (40.5%) and 23 (62.2%) were positive for the SVA in the RT-PCR and nested-PCR assays, respectively. The SVA RNA was detected in 61/177 (34.5%) samples with the RT-PCR, while the nested-PCR assay showed 84/177 (47.5%) samples with the virus (p < 0.05). According to the herds, 11 (61.1%) and 16 (88.9%) of the 18 pig herds were positive for the SVA in the RT-PCR and nested-PCR assays, respectively. Nucleotide sequencing analysis revealed similarities of 98.7-100% among SVA Brazilian strains and of 86.6-98% with SVA strains from other countries. The nested-PCR assay in this study was suitable to recover the SVA RNA in biological specimens, piglets, and/or herds that were considered as negative in the RT-PCR assay, and is proposed for the routine investigation of the SVA infection in piglets, especially when other techniques are not available or when a great number of samples has to be examined.
A组赛内卡病毒（SVA）与断奶仔猪和成年猪的水疱性疾病有关，也与新生仔猪的高死亡率有关。本研究旨在建立一种巢式PCR检测方法，用于SVA感染的常规诊断。从巴西四个不同州的18个猪场的37头仔猪中采集了组织样本（n = 177）。对于巢式PCR，定义了一组引物，用于扩增SVA基因组316 bp的内部VP1片段。在37头仔猪中，RT-PCR和巢式PCR检测分别有15头（40.5%）和23头（62.2%）SVA呈阳性。RT-PCR在61/177（34.5%）的样本中检测到SVA RNA，而巢式PCR检测显示有84/177（47.5%）的样本含有该病毒（p < 0.05）。根据猪群情况，18个猪群中分别有11个（61.1%）和16个（88.9%）在RT-PCR和巢式PCR检测中SVA呈阳性。核苷酸序列分析显示，巴西SVA毒株之间的相似性为98.7%-100%，与其他国家的SVA毒株的相似性为86.6%-98%。本研究中的巢式PCR检测方法适用于从RT-PCR检测中被视为阴性的生物样本、仔猪和/或猪群中检测出SVA RNA，建议将其用于仔猪SVA感染的常规调查，特别是在没有其他可用技术或需要检测大量样本时。
Zotero 插件