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[激光能量照射后尿路上皮的形态学检查]

[Morphologic examination of the urothelium after irradiation with laser energy].

作者信息

Hofmann R, Hartung R, Geissdörfer K, Ascherl R, Erhardt W, Schmidt-Kloiber H, Reichel E, Schöffmann H

机构信息

Urologische Klinik und Poliklinik, TU München, BRD.

出版信息

Urol Int. 1987;42(6):434-7. doi: 10.1159/000282010.

Abstract

The energy of a Nd-YAG laser (1,064 nm wave length, 8 ns pulse duration) was used to irradiate the urothelium of the ureter or bladder and kidney parenchyma in pigs. Single pulse energy was 50-120 mJ with a 20-Hz repetition rate. The horizontal laser beam was reflected 90 degrees down by a 100% mirror and with a specially designed apparatus focussed on the surface of the tissue. Laser light from a quartz glass fiber was also focussed directly onto the tissue. Urothelium and kidney parenchyma were irradiated in 7 pigs. Tissue samples were examined histologically and raster electron microscopically 2, 4, 8 and 12 days after irradiation. No macroscopic lesion could be found. Maximum energy caused a small cone of 40 micron depth. No thermic effects or necrosis resulted, so that no harm is to be expected with unintentional irradiation.

摘要

使用钕钇铝石榴石激光(波长1064纳米,脉冲持续时间8纳秒)的能量照射猪的输尿管、膀胱或肾脏实质的尿路上皮。单脉冲能量为50 - 120毫焦,重复频率为20赫兹。水平激光束由一个100%的反射镜向下反射90度,并通过一个专门设计的装置聚焦在组织表面。来自石英玻璃纤维的激光也直接聚焦在组织上。对7头猪的尿路上皮和肾脏实质进行了照射。在照射后2、4、8和12天对组织样本进行了组织学和扫描电子显微镜检查。未发现肉眼可见的病变。最大能量造成了一个深度为40微米的小锥体。未产生热效应或坏死,因此意外照射预计不会造成伤害。

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