REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, no 228, 4050-313, Portugal; CIMO-ESA, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 1172, 5301-855 Bragança, Portugal.
CIMO-ESA, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 1172, 5301-855 Bragança, Portugal; CIBIO-ICETA, Faculdade de Ciências, Universidade do Porto, R. Padre Armando Quintas, 4485-661 Vairão, Portugal.
Food Res Int. 2017 Oct;100(Pt 3):517-523. doi: 10.1016/j.foodres.2016.07.009. Epub 2016 Jul 20.
There is an increasing trend towards finding alternative sources of valued phytochemicals due to their diverse potentialities in food industry and pharmaceutical applications. Phenolic compounds, in particular, have been the focus of several profiling reports, but isoflavones characterization has been studied in fewer cases and in a very limited group of plant species. Despite their acknowledged bioactivity, there's actually a strict number of plants validated for their isoflavones contents. In a previous report, we have identified nine Leguminosae species (from genera Biserrula, Lotus, Ornithopus and Scorpiurus) as potential alternative sources of these phenolic compounds. However, the isoflavone profiles are highly modulated by the ontogenic stage. Therefore, the present study was conducted in the same Leguminosae species, but harvested at three sequential vegetative development stages: vegetative elongation, late bud and late flowering, with the main purpose of assessing the evolution of isoflavones content throughout the plant development. In general, the plant species from Biserrula and Lotus genera showed the highest potential as new natural sources of isoflavones, especially owing their high levels of biochanin A. Independently of the plant species, it was possible to identify the phenologic stages where each of the quantified isoflavones is maximized. These findings are useful to predict isoflavone yields according to harvesting time, validating the potential use of the studied plants in innovative food formulations.
由于其在食品工业和制药应用中的多样化潜力，人们越来越倾向于寻找有价值的植物化学物质的替代来源。特别是酚类化合物，已经成为了多个分析报告的焦点，但异黄酮的特征研究则较少，而且仅限于非常有限的植物物种。尽管它们的生物活性得到了公认，但实际上只有少数几种植物因其异黄酮含量而得到了验证。在之前的一份报告中，我们已经确定了九种豆科植物（来自双柱柳属、百脉根属、鹅观草属和 Scorpio 属）作为这些酚类化合物的潜在替代来源。然而，异黄酮的种类受到个体发育阶段的高度调节。因此，本研究在相同的豆科植物中进行，但在三个连续的营养生长阶段进行收获：营养伸长、晚期芽和晚期开花，主要目的是评估异黄酮含量在整个植物发育过程中的变化。一般来说，来自双柱柳属和百脉根属的植物表现出作为异黄酮新的天然来源的最大潜力，特别是因为它们具有高水平的大豆苷元 A。独立于植物物种，可以确定每个定量异黄酮达到最大值的物候阶段。这些发现有助于根据收获时间预测异黄酮产量，验证所研究植物在创新食品配方中的潜在用途。
