The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
Fish Shellfish Immunol. 2017 Dec;71:76-82. doi: 10.1016/j.fsi.2017.09.064. Epub 2017 Sep 28.
Hepatic steatosis induced inflammation is becoming increasingly prevalent in farmed fish. This study was conducted to investigate the protective effects of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) against hepatic steatosis-induced inflammation and its potential molecular mechanisms in hepatocyte of large yellow croaker (Larmichthys crocea). We found that the hepatic steatosis-induced inflammation was relieved by ω-3 PUFAs, meanwhile, the Sirt1 activity and transcript expression was increased by ω-3 PUFAs. The increased Sirt1 activity can decrease the hepatic steatosis-induced inflammation. The protective effects of ω-3 PUFAs against hepatic steatosis-induced inflammation was reversed by the treatment with Sirt1 inhibitor EX-527. The nuclear translocation of nuclear transcription factor kappa-B (NF-κB) p65 was significantly decreased after ω-3 PUFAs treatments compared to the palmitic acid stimulation group. The ω-3 PUFAs induced cytoplasm translocation of NF-κB p65 was reversed by EX-527. Together, ω-3 PUFAs alleviate hepatic steatosis-induced inflammation through Sirt1-mediated nuclear translocation of NF-κB p65 subunit in hepatocytes of large yellow croaker. The present study provides important insight into the mechanisms of the protective effects of ω-3 PUFAs, providing theory bases for alleviating the hepatic steatosis induced inflammation of farmed fish, thereby offering great benefits to the aquaculture industry and fish consumers.
养殖鱼类的肝脂肪变性诱导炎症日益普遍。本研究旨在探讨ω-3 多不饱和脂肪酸（ω-3 PUFAs）对大黄鱼（Larmichthys crocea）肝细胞肝脂肪变性诱导炎症的保护作用及其潜在的分子机制。我们发现，ω-3 PUFAs 缓解了肝脂肪变性诱导的炎症，同时，ω-3 PUFAs 增加了 Sirt1 活性和转录表达。增加的 Sirt1 活性可以减少肝脂肪变性诱导的炎症。用 Sirt1 抑制剂 EX-527 处理可逆转 ω-3 PUFAs 对肝脂肪变性诱导的炎症的保护作用。与软脂酸刺激组相比，ω-3 PUFAs 处理后核转录因子 kappa-B（NF-κB）p65 的核易位明显减少。EX-527 逆转了 ω-3 PUFAs 诱导的 NF-κB p65 胞质易位。总之，ω-3 PUFAs 通过 Sirt1 介导的大黄鱼肝细胞 NF-κB p65 亚基的核易位缓解肝脂肪变性诱导的炎症。本研究为 ω-3 PUFAs 的保护作用机制提供了重要的见解，为缓解养殖鱼类肝脂肪变性诱导的炎症提供了理论依据，从而为水产养殖业和鱼类消费者带来了巨大的利益。
