猫E3泛素连接酶BCA2的自泛素化

Autoubiquitination of feline E3 ubiquitin ligase BCA2.

作者信息

Wang Weiran, Qu Meng, Wang Jiawen, Zhang Xin, Zhang Haihong, Wu Jiaxin, Yu Bin, Wu Hui, Kong Wei, Yu Xianghui

机构信息

National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin Province, People's Republic of China.

National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin Province, People's Republic of China; Center for New Medicine Research, Changchun University of Chinese Medicine, Changchun, Jilin Province, People's Republic of China.

出版信息

Gene. 2018 Jan 5;638:1-6. doi: 10.1016/j.gene.2017.09.059. Epub 2017 Sep 28.

DOI:10.1016/j.gene.2017.09.059

PMID:28964893

Abstract

BCA2/RNF115/Rabring7 is a RING type E3 ubiquitin ligase that is overexpressed in human breast tumors and is important for regulating breast cancer cell migration. In the present investigation, feline BCA2 (fBCA2) was identified and characterized. Compared with its human counterpart, the fBCA2 cDNA was confirmed to be 918 base pairs in length showing 92.6% consensus and identity positions, encoding a protein of 305 amino acids with 96.7% consensus and 93.1% identity positions. The fBCA2 protein contains a RING domain at the C-terminus, which was found to be essential for its autoubiquitination.

摘要

BCA2/RNF115/Rabring7是一种RING型E3泛素连接酶，在人类乳腺肿瘤中过表达，对调节乳腺癌细胞迁移很重要。在本研究中，鉴定并表征了猫BCA2（fBCA2）。与其人类对应物相比，fBCA2 cDNA经确认长度为918个碱基对，共有序列和相同位置显示为92.6%，编码一个由305个氨基酸组成的蛋白质，共有序列为96.7%，相同位置为93.1%。fBCA2蛋白在C端含有一个RING结构域，发现该结构域对其自身泛素化至关重要。