Department of Obstetrics and Gynecology, Dokuz Eylül University Medical School Hospital, İzmir, Turkey.
Department of Medical Biology and Genetics, Dokuz Eylül University Medical School Hospital, İzmir, Turkey.
Fertil Steril. 2013 Jan;99(1):264-269.e3. doi: 10.1016/j.fertnstert.2012.08.059. Epub 2012 Oct 1.
To evaluate the levels of hepatocyte growth factor (HGF) in follicular fluid (FF) and the expression of c-Met in granulosa cells (GCs) with respect to the quality of the oocyte and embryo both in patients with polycystic ovary syndrome (PCOS) and in the normal ovary during controlled ovarian hyperstimulation cycles.
Prospective controlled study.
University hospital.
PATIENT(S): Fifty-nine women undergoing IVF treatment (of whom 21 had PCOS and 38 were in the control group).
INTERVENTION(S): A total of 168 FF samples were collected at the time of oocyte retrieval. The HGF levels were measured by ELISA, and the mRNA expression of c-Met in GCs was detected by real-time polymerase chain reaction.
MAIN OUTCOME MEASURE(S): The predictive values of HGF levels in serum and FF and the mRNA expression of c-Met in GCs for successful fertilization and oocyte-embryo quality.
RESULT(S): The levels of HGF in serum and FF and the c-Met expression in GCs were similar between the PCOS and control groups. Granulosa cells of fertilized oocytes (2PN) had a significantly higher level of c-Met expression than that in oocytes that failed to fertilize. The mean HGF level in FF was significantly higher in the grade 1 embryos than in the grades 2-4 embryos.
CONCLUSION(S): This study suggests that HGF/c-Met signaling may be a crucial determinant of fertilization success.
评估多囊卵巢综合征（PCOS）患者和正常卵巢控制性卵巢刺激周期中卵母细胞和胚胎质量与卵泡液（FF）中肝细胞生长因子（HGF）水平和颗粒细胞（GC）中 c-Met 表达的关系。
前瞻性对照研究。
大学医院。
59 名接受 IVF 治疗的妇女（其中 21 名患有 PCOS，38 名是对照组）。
在取卵时共采集 168 个 FF 样本。通过 ELISA 测量 HGF 水平，通过实时聚合酶链反应检测 GC 中 c-Met 的 mRNA 表达。
血清和 FF 中 HGF 水平和 GC 中 c-Met mRNA 表达对受精和卵-胚胎质量的预测价值。
PCOS 组和对照组血清和 FF 中的 HGF 水平以及 GC 中的 c-Met 表达相似。受精的 2 原核（2PN）卵的 GC 中 c-Met 表达水平明显高于未受精的卵。FF 中的 HGF 平均水平在 1 级胚胎中明显高于 2-4 级胚胎。
本研究表明 HGF/c-Met 信号通路可能是受精成功的关键决定因素。
