Department of Thyroid and Breast, Huaihe Hospital, Henan University, Kaifeng 475000, People's Republic of China.
Department of Endocrinology, Huaihe Hospital, Henan University, Kaifeng 475000, People's Republic of China.
Biomed Pharmacother. 2017 Dec;96:98-103. doi: 10.1016/j.biopha.2017.09.132. Epub 2017 Sep 29.
Tripartite motif 44 (TRIM44), a member of the TRIM family, functions as a critical regulator in several types of malignancy. However, the role of TRIM44 in thyroid cancer has never been revealed. Thus, in this study, to explore its role in PTC, we detected its expression patterns in human papillary thyroid cancer (PTC) tissues and cell lines, and investigated its effects on cell proliferation and invasion. Our results demonstrated that TRIM44 was highly expressed in human PTC tissues and cell lines. In addition, silencing of TRIM44 significantly suppressed the proliferation, migration/invasion, and the epithelial-mesenchymal transition (EMT) process in PTC cells. Furthermore, silencing of TRIM44 dramatically down-regulated the expression of β-catenin, cyclin-D1 and c-Myc in PEC cells, and the activator of the Wnt/β-catenin pathway LiCl rescued the anticancer effect of knockdown TRIM44 expression in PTC cells. In conclusion, this study reported here provided evidence that TRIM44 functions as an oncogene in PTC. Silencing of TRIM44 inhibits the proliferation, migration and invasion of PTC cells in part through suppression of the Wnt/β-catenin signaling pathway. Thus, TRIM44 may be a potential therapeutic target for the treatment of PTC.
三结构域蛋白 44（TRIM44）是 TRIM 家族的成员，在几种类型的恶性肿瘤中作为关键调节因子发挥作用。然而，TRIM44 在甲状腺癌中的作用从未被揭示。因此，在这项研究中，为了探索其在 PTC 中的作用，我们检测了其在人甲状腺乳头状癌（PTC）组织和细胞系中的表达模式，并研究了其对细胞增殖和侵袭的影响。我们的研究结果表明，TRIM44 在人 PTC 组织和细胞系中高度表达。此外，沉默 TRIM44 显著抑制 PTC 细胞的增殖、迁移/侵袭和上皮-间充质转化（EMT）过程。此外，沉默 TRIM44 可显著下调 PEC 细胞中β-连环蛋白、细胞周期蛋白 D1 和 c-Myc 的表达，Wnt/β-连环蛋白通路的激活剂 LiCl 挽救了敲低 TRIM44 表达对 PTC 细胞的抗癌作用。总之，本研究报道了 TRIM44 作为 PTC 中的癌基因的证据。沉默 TRIM44 可部分通过抑制 Wnt/β-连环蛋白信号通路抑制 PTC 细胞的增殖、迁移和侵袭。因此，TRIM44 可能是治疗 PTC 的潜在治疗靶点。
