Instituto de Química, Departamento de Productos Naturales, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Delegación Coyoacán, Ciudad de México, 04510, México.
Appl Microbiol Biotechnol. 2017 Nov;101(22):8209-8222. doi: 10.1007/s00253-017-8542-8. Epub 2017 Sep 30.
To assess their potential as biopesticides, the effect on the growth of phytopathogen Fusarium oxysporum of six volatile organic compounds from endophytic fungi was studied in vivo and in vitro; compounds were used both as a mixture and individually. In vivo studies were performed inoculating the pathogen into cherry tomatoes, while the in vitro antifungal effect was studied using agar dilution and gas phase methods. Also, the morphology of the hyphae exposed to these compounds was analyzed. Moreover, the possible mechanism of action of these compounds was determined by studying the respiration and cell membrane permeability. Results show that the compounds have a significant concentration-dependent antifungal effect individually and act in a synergic manner. Additionally, changes in cell membrane permeability, damage to the hyphal morphology, and an inhibitory effect on the respiration were observed. The mixture of the six compounds may be used for postharvest control of F. oxysporum in tomatoes.
为了评估它们作为生物农药的潜力，研究了来自内生真菌的六种挥发性有机化合物对植物病原菌尖孢镰刀菌生长的影响，无论是在体内还是体外，这些化合物都被用作混合物或单独使用。体内研究是通过将病原体接种到樱桃番茄中进行的，而体外抗真菌效果则使用琼脂稀释和气相方法进行研究。此外，还分析了暴露于这些化合物的菌丝的形态。此外，通过研究呼吸作用和细胞膜通透性来确定这些化合物的可能作用机制。结果表明，这些化合物具有显著的浓度依赖性的单独的抗真菌作用，并具有协同作用。此外，观察到细胞膜通透性的变化、菌丝形态的损伤以及对呼吸作用的抑制作用。这六种化合物的混合物可用于番茄中尖孢镰刀菌的采后控制。
