Centro de Investigaciones en Fitopatología (CIDEFI), La Plata, Argentina.
Comisión de Investigaciones Científicas de La Provincia de Buenos Aires (CICBA), La Plata, Argentina.
Arch Microbiol. 2021 May;203(4):1383-1397. doi: 10.1007/s00203-020-02136-y. Epub 2021 Jan 2.
The endophytic microbiome uses mechanisms such as the secretion of diffusible antibiotic molecules, synthesis and release of volatile organic compounds, and/or toxins to protect plants. The aim of this research was to study the volatile organic compounds (VOCs) profile as well as the diffusible secondary metabolites produced and released by endophytic bacteria isolated from tomato plants that in in-vitro assays prevented growth of pathogenic fungi. Bacteria belonging to seven genera (Acinetobacter, Arthrobacter, Bacillus, Microbacterium, Pantoea, Pseudomonas, and Stenotrophomonas) were isolated from different tissues of tomato plants with and without symptoms of Gray leaf spot, a disease provoked by Stemphylium lycopersici. In vitro, antagonistic assays were performed and the effect of volatile and soluble compounds released by endophytic bacteria on the growth of pathogenic fungi was determined. The VOCs synthesized by the endophytes were extracted, identified and quantified. These isolates representatives of seven bacterial genera inhibited the growth of three fungal pathogens of tomato S. lycopersici, Alternaria alternata and Corynespora cassiicola, which was related to the synthesis of soluble compounds as well as VOCs. Endophytes synthesize and release different VOCs, probably due to the different type of interaction that each bacterium establishes with the fungus, presenting a range of fungal growth inhibition.
内生微生物群落利用分泌可扩散抗生素分子、合成和释放挥发性有机化合物和/或毒素等机制来保护植物。本研究旨在研究从番茄植物中分离出的内生细菌产生和释放的挥发性有机化合物 (VOC) 谱以及可扩散次生代谢物，这些内生细菌在体外试验中可防止病原菌真菌的生长。从有和无症状感染灰斑病（由链格孢引起的疾病）的番茄植物的不同组织中分离出属于七个属（不动杆菌属、节杆菌属、芽孢杆菌属、微杆菌属、泛菌属、假单胞菌属和寡养单胞菌属）的细菌。在体外进行拮抗测定，确定内生细菌释放的挥发性和可溶性化合物对病原菌真菌生长的影响。提取、鉴定和定量了内生菌合成的 VOCs。这些内生细菌的代表菌株抑制了番茄病原菌灰斑病菌、Alternaria alternata 和 Corynespora cassiicola 的生长，这与可溶性化合物以及 VOCs 的合成有关。内生菌合成和释放不同的 VOCs，可能是由于每种细菌与真菌建立的不同类型的相互作用，表现出一系列抑制真菌生长的作用。
