Sun Hai-Bo, Wang Min, Liu Yuan-Zhong, Dang Rong-Min, Xie Hong-Shu, Li Zhang-Chun
Department of Physical Education,Qiannan Medical College for Nationalities, Duyun, Guizhou 558003, China.
Department of Pathology, Qiannan Medical College for Nationalities, Duyun, Guizhou 558003, China.
Zhonghua Nan Ke Xue. 2017 Jan;23(1):21-26.
To observe the effects of swimming plus medication on the expressions of cytokines in rats with chronic abacterial prostatitis (CAP).
Forty healthy adult male SD rats were randomly divided into five groups of equal number, normal control, CAP model control, medication, exercise therapy, and exercise + medication. The CAP model was made by Xiaozhiling injection, and at 7 days after modeling, the rats in the medication and exercise + medication groups were treated intragastrically with Qianlie Shutong Capsules (0.016 g/ml) at 20 ml per kg of the body weight qd, those in the exercise therapy and exercise + medication groups were made swim at a regular time once a day, 35 minutes on the first day and 5 minutes more on the second until 50 minutes once, for 4 successive weeks, and those in the normal control, model control and exercise therapy groups received normal saline only. After 14 and 28 days of treatment, all the rats were killed and their prostates harvested for observation of histopathological changes and determination of the expressions of TNF- α, IL-1β and IL-6 in the prostatic tissue homogenate by ELISA.
After 14 days of treatment, the expression levels of TNF-α, IL-1β and IL-6 were significantly elevated in the groups of CAP model control (［183.08±8.07］ pg/ml, ［57.55±3.53］ pg/ml and ［256.15±13.95］ ng/L), medication (［118.49±8.06］ pg/ml, ［42.64±4.64 ］ pg/ml and ［200.74±9.33］ ng/L), exercise therapy (［169.63±10.64］ pg/ml, ［50.45±5.71］ pg/ml and ［245.23±6.49］ ng/L), and exercise + medication (［107.82±7.81］ pg/ml, ［40.35±6.93］ pg/ml and ［187.04±10.85］ ng/L) as compared with those in the normal control (［20.36±1.82］ pg/ml, ［14.64±1.91］ pg/ml and ［70.58±2.09］ ng/L) (P<0.05). At 28 days, the levels of TNF- α, IL-1β, IL-6 were remarkably lower in the exercise + medication group (［29.30±3.78］ pg/ml, ［16.91±1.24］ pg/ml and ［ 88.65±6.74］ ng/L) than in the medication group (［39.67±3.19］ pg/ml, ［26.27±3.49］ pg/ml and ［110.26±6.33］ ng/L) (P<0.05) and close to those of the normal control group (［19.34±1.76］ pg/ml, ［13.68±1.06］ pg/ml and ［71.34±2.50］ ng/L). During the treatment, no obvious pathological changes were found in the prostate tissue of the normal control rats, while significant chronic prostatic inflammation was observed in the CAP models, and the inflammation was relieved in different degrees after intervention, most significantly in the exercise + medication group.
Swimming can relieve prostatic inflammation and swimming plus medication can effectively reduce the expressions of cytokines and alleviate histological damage in the prostatic tissue of CAP rats.
观察游泳联合药物治疗对慢性非细菌性前列腺炎（CAP）大鼠细胞因子表达的影响。
将40只健康成年雄性SD大鼠随机分为5组，每组数量相等，即正常对照组、CAP模型对照组、药物治疗组、运动治疗组和运动+药物治疗组。采用消痔灵注射液制备CAP模型，造模后7天，药物治疗组和运动+药物治疗组大鼠按20 ml/kg体重灌胃前列舒通胶囊（0.016 g/ml），每日1次；运动治疗组和运动+药物治疗组大鼠每天定时游泳1次，第1天35分钟，第2天开始每次增加5分钟，直至每次50分钟，连续4周；正常对照组、模型对照组和运动治疗组大鼠仅给予生理盐水。治疗14天和治疗28天后，处死所有大鼠，摘取前列腺，观察组织病理学变化，并采用酶联免疫吸附测定法（ELISA）检测前列腺组织匀浆中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的表达。
治疗14天后，CAP模型对照组（［183.08±8.07］ pg/ml、［57.55±3.53］ pg/ml、［256.15±13.95］ ng/L）、药物治疗组（［118.49±8.06］ pg/ml、［42.64±4.64］ pg/ml、［200.74±9.33］ ng/L）、运动治疗组（［169.63±10.64］ pg/ml、［50.45±5.71］ pg/ml、［245.23±6.49］ ng/L）和运动+药物治疗组（［107.82±7.81］ pg/ml、［40.35±6.93］ pg/ml、［187.04±10.85］ ng/L）中TNF-α、IL-1β和IL-6的表达水平均显著高于正常对照组（［20.36±1.82］ pg/ml、［14.64±1.91］ pg/ml、［70.58±2.09］ ng/L）（P<0.05）。治疗28天后，运动+药物治疗组中TNF-α、IL-1β、IL-6的水平（［29.30±3.78］ pg/ml、［16.91±1.24］ pg/ml、［88.65±6.74］ ng/L）显著低于药物治疗组（［39.67±3.19］ pg/ml、［26.27±3.49］ pg/ml、［110.26±6.33］ ng/L）（P<0.05），且接近正常对照组水平（［19.34±1.76］ pg/ml、［13.68±1.06］ pg/ml、［71.34±2.50］ ng/L）。治疗期间，正常对照组大鼠前列腺组织未发现明显病理变化，而CAP模型大鼠出现明显慢性前列腺炎，干预后炎症均有不同程度缓解，其中运动+药物治疗组缓解最为明显。
游泳可缓解前列腺炎症，游泳联合药物治疗能有效降低CAP大鼠前列腺组织中细胞因子的表达，减轻组织学损伤。
