Cai Wen-Wei, Mo Dun-Sheng, Fan Ming, Cai Hong-Cai, Zhang Guo-Wei, Wang Wei-Piong, Shang Xue-Jun
Center of Reproductive Medicine, Jiaxing Women and Children's Hospital, Jiaxing, Zhejiang 314000, China.
Department of Urology, Liuzhou Workers' Hospital / The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, China.
Zhonghua Nan Ke Xue. 2018 Jun;24(6):491-498.
To investigate the effects of fosfomycin tromethamine (FT) on the expressions of tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), and interleukin-6 (IL-6) in the prostate tissue of the rats with chronic bacterial prostatitis (CBP).
We randomly divided 70 male SD rats into 7 groups of equal number: blank control, CBP model control, positive control, 14 d low-dose FT, 7 d low-dose FT, 14 d high-dose FT, and 7 d high-dose FT. The CBP model rats in the latter five groups were treated intragastrically with levofloxacin at 100 mg/kg/d for 30 days and FT at 200 mg/kg/d for 14 and 7 days and at 300 mg/kg/d for 14 and 7 days, respectively. Then we collected the prostate tissue from the animals for determination of the levels of TNF-α, IL-8 and IL-6 by ELISA.
Compared with the blank controls, the CBP model rats showed significantly increased levels of TNF-α (［19.83 ± 6.1］ vs ［32.93 ± 6.21］ ng/g prot, P <0.01), IL-8 (［8.26 ± 0.52］ vs ［16.2 ± 2.84］ ng/g prot, P <0.01) and IL-6 (［1.55 ± 0.11］ vs ［2.51 ± 1.06］ ng/g prot, P <0.05) in the prostate tissue. In comparison with the CBP model controls, the levels of TNF-α and IL-8 were remarkably decreased in the groups of positive control (［20.54 ± 5.78］ ng/g prot, P <0.01; ［12.43 ± 4.02］ ng/g prot, P <0.05), 14 d low-dose FT (［21.95 ± 6.48］ ng/g prot, P <0.01; ［11.11 ± 2.86］ ng/g prot, P <0.01), 7 d low-dose FT (［23.8 ± 6.93］ ng/g prot, P <0.05; ［12.43 ± 4.02］ ng/g prot, P <0.05), 14 d high-dose FT (［19.97 ± 2.58］ ng/g prot, P <0.01; ［8.83 ± 1.32］ ng/g prot, P <0.01), and 7 d high-dose FT (［21.97 ± 3.38］ ng/g prot, P <0.01; ［12.68±1.97］ ng/g prot, P <0.05). No statistically significant differences were observed between the positive control and FT groups in the contents of TNF-α, IL-8 or IL-6 (P >0.05). The expression of IL-6 was markedly reduced in the 14 d high-dose FT group as compared with the model controls (［1.76 ± 0.46］ vs ［2.51 ± 1.06］ ng/g prot, P<0.05) but exhibited no significant difference between the CBP model control and the other groups (P >0.05).
Fosfomycin tromethamine inhibits the expressions of TNF-α, IL-8 and IL-6 in the prostate tissue, suppresses its inflammatory reaction, promotes the repair of damaged prostatic structure, and thus contributes to the treatment of chronic bacterial prostatitis in rats.
探讨磷霉素氨丁三醇（FT）对慢性细菌性前列腺炎（CBP）大鼠前列腺组织中肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）和白细胞介素-6（IL-6）表达的影响。
将70只雄性SD大鼠随机分为7组，每组数量相等：空白对照组、CBP模型对照组、阳性对照组、14天低剂量FT组、7天低剂量FT组、14天高剂量FT组和7天高剂量FT组。后五组的CBP模型大鼠分别以100 mg/kg/d的左氧氟沙星灌胃30天，以200 mg/kg/d的FT灌胃14天和7天，以及以300 mg/kg/d的FT灌胃14天和7天。然后收集动物的前列腺组织，通过酶联免疫吸附测定法（ELISA）测定TNF-α、IL-8和IL-6的水平。
与空白对照组相比，CBP模型大鼠前列腺组织中TNF-α（［19.83±6.1］对［32.93±6.21］ng/g蛋白，P<0.01）、IL-8（［8.26±0.52］对［16.2±2.84］ng/g蛋白，P<0.01）和IL-6（［1.55±0.11］对［2.51±1.06］ng/g蛋白，P<0.05）的水平显著升高。与CBP模型对照组相比，阳性对照组（［20.54±5.78］ng/g蛋白，P<0.01；［12.43±4.02］ng/g蛋白，P<0.05）、14天低剂量FT组（［21.95±6.48］ng/g蛋白，P<0.01；［11.11±2.86］ng/g蛋白，P<0.01）、7天低剂量FT组（［23.8±6.93］ng/g蛋白，P<0.05；［12.43±4.02］ng/g蛋白，P<0.05）、14天高剂量FT组（［19.97±2.58］ng/g蛋白，P<0.01；［8.83±1.32］ng/g蛋白，P<0.01）和7天高剂量FT组（［21.97±3.38］ng/g蛋白，P<0.01；［12.68±1.97］ng/g蛋白，P<0.05）中TNF-α和IL-8的水平显著降低。阳性对照组与FT组在TNF-α、IL-8或IL-6含量方面未观察到统计学显著差异（P>0.05）。与模型对照组相比，14天高剂量FT组中IL-6的表达显著降低（［1.76±0.46］对［2.51±1.06］ng/g蛋白，P<0.05），但在CBP模型对照组与其他组之间未表现出显著差异（P>0.05）。
磷霉素氨丁三醇抑制前列腺组织中TNF-α、IL-8和IL-6的表达，抑制其炎症反应，促进受损前列腺结构的修复，从而有助于治疗大鼠慢性细菌性前列腺炎。
