Non-Viral Gene Delivery Laboratory, Discipline of Biochemistry, School of Life Sciences, University of Kwa-Zulu Natal, Private Bag X54001, Durban, Kwa-Zulu Natal, South Africa.
Nanomedicine (Lond). 2017 Nov;12(21):2625-2640. doi: 10.2217/nnm-2017-0228. Epub 2017 Oct 2.
To synthesize and functionalize platinum (core)-gold (shell) bimetallic nanoparticles (PtAuBNps) with chitosan and doxorubicin to display favorable pharmacokinetics, biodegradability, biological activity and safety in vitro.
MATERIALS & METHODS: PtAuBNps and their drug nanocomposites were morphologically and physico-chemically characterized. Binding studies determined the efficiency and stability of the platform. In vitro release kinetics were evaluated under simulated environments, cytotoxicity profiles through MTT and Sulforodhamine B assays and apoptosis induction using the dual EtBr/AO staining.
RESULTS & DISCUSSION: The results obtained indicate that functionalized PtAuBNps displayed favorable physio-chemical attributes, high binding capabilities, pH-triggered drug release through zero-order release kinetics, cell-specific cytotoxicity and good colloidal stability.
The positive attributes of this novel delivery system bodes well for future in vivo studies.
用壳聚糖和阿霉素对铂（核）-金（壳）双金属纳米粒子（PtAuBNps）进行合成和功能化，以显示出有利的药代动力学、生物降解性、体外生物活性和安全性。
对 PtAuBNps 及其药物纳米复合物进行了形态和物理化学特性的表征。结合研究确定了该平台的效率和稳定性。在模拟环境下评估了体外释放动力学，通过 MTT 和 Sulforodhamine B 测定法评估细胞毒性谱，并用双重 EtBr/AO 染色诱导细胞凋亡。
结果表明，功能化的 PtAuBNps 表现出良好的物理化学特性、高结合能力、通过零级释放动力学的 pH 触发药物释放、细胞特异性细胞毒性和良好的胶体稳定性。
这种新型递药系统的积极属性为未来的体内研究提供了良好的前景。
