N-乙酰-4-氧代-D-神经氨酸对N-乙酰神经氨酸裂解酶的抑制作用。

Inhibition of N-acetylneuraminate lyase by N-acetyl-4-oxo-D-neuraminic acid.

作者信息

Gross H J, Brossmer R

机构信息

Institut für Biochemie II, Universität Heidelberg, FRG.

出版信息

FEBS Lett. 1988 May 9;232(1):145-7. doi: 10.1016/0014-5793(88)80404-6.

DOI:10.1016/0014-5793(88)80404-6

PMID:2896604

Abstract

We show that the 4-oxo analogue of N-acetyl-D-neuraminic acid strongly inhibits N-acetylneuraminate lyase (NeuAc aldolase, EC 4.1.3.3) from Clostridum perfringens (Ki = 0.025 mM) and Escherichia coli (Ki = 0.15 mM). In each case the inhibition was competitive. N-Acetyl-D-neuraminic acid; N-Acetylneuraminate lyase; N-Acetyl-D-neuraminic acid analog; 5-Acetamido-3,5-dideoxy-beta-D-manno-non-2,4-diulosonic acid; 2-Deoxy-2,3-didehydro-N-acetyl-4-oxo-neuraminic acid; Competitive inhibitor.

摘要

我们发现，N-乙酰-D-神经氨酸的4-氧代类似物能强烈抑制产气荚膜梭菌（Ki = 0.025 mM）和大肠杆菌（Ki = 0.15 mM）中的N-乙酰神经氨酸裂解酶（神经氨酸醛缩酶，EC 4.1.3.3）。在每种情况下，抑制作用均为竞争性。N-乙酰-D-神经氨酸；N-乙酰神经氨酸裂解酶；N-乙酰-D-神经氨酸类似物；5-乙酰氨基-3,5-二脱氧-β-D-甘露糖壬-2,4-二酮糖酸；2-脱氧-2,3-二脱氢-N-乙酰-4-氧代神经氨酸；竞争性抑制剂。