免疫系统疾病中的记忆淋巴细胞免疫刺激试验：有用还是无用？
The memory lymphocyte immunostimulation assay in immune system disorders: Is useful or useless?
作者信息
Vadalà Maria, Laurino Carmen, Palmieri Beniamino
机构信息
Department of General Surgery and Surgical Specialties, University of Modena and Reggio Emilia Medical School, Surgical Clinic, Modena, Italy.
Network of the Second Opinion, Modena, Italy.
出版信息
J Lab Physicians. 2017 Oct-Dec;9(4):223-226. doi: 10.4103/JLP.JLP_137_16.
AIM
The aim of the study was to evaluate the clinical relevance, sensitivity and specificity of blood test, Memory Lymphocyte ImmunoStimulation Assay (MELISA), in genetically predisposed patients that suffer by autoimmune/inflammatory syndrome induced by adjuvants, after HPV-vaccination and that could have a high metal hypersensitivity.
MATERIALS AND METHODS
Sixteen girls (aged 12-24 years) that developed long-lasting and invalidating somatoform symptoms occurring within 20 days postvaccination are included in this descriptive study. The hypersensitivity to five metals (aluminum, nickel, mercury, methyl mercury, and thimerosal) was measured by MELISA test.
RESULTS
Seven girls showed negativity to all the five metals tested. The findings showed metal hypersensitivity only in nine patients: Toxicity to aluminum (two girls), reactivity to nickel (seven girls), followed by mercury (seven girls).
CONCLUSION
The MELISA assay is neither sensitive nor specific in detecting metal hypersensitivity and associated chronic diseases, including autoimmune pathologies.
目的
本研究旨在评估血液检测——记忆淋巴细胞免疫刺激试验（MELISA）在遗传易感性患者中的临床相关性、敏感性和特异性。这些患者在接种人乳头瘤病毒（HPV）疫苗后患有佐剂诱导的自身免疫/炎症综合征，且可能对金属高度过敏。
材料与方法
本描述性研究纳入了16名女孩（年龄在12至24岁之间），她们在接种疫苗后20天内出现了持续且使人衰弱的躯体形式症状。通过MELISA试验检测对五种金属（铝、镍、汞、甲基汞和硫柳汞）的过敏情况。
结果
7名女孩对所有检测的五种金属均呈阴性反应。结果显示仅9名患者存在金属过敏：对铝中毒（2名女孩），对镍有反应（7名女孩），其次是对汞有反应（7名女孩）。
结论
MELISA试验在检测金属过敏及相关慢性疾病（包括自身免疫性疾病）方面既不敏感也不具有特异性。
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