Keikhosravi Adib, Liu Yuming, Drifka Cole, Woo Kaitlin M, Verma Amitabh, Oldenbourg Rudolf, Eliceiri Kevin W
Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison WI, USA.
Biomedical Engineering Department, University of Wisconsin at Madison, Madison WI, USA.
Biomed Opt Express. 2017 Aug 29;8(9):4243-4256. doi: 10.1364/BOE.8.004243. eCollection 2017 Sep 1.
A number of histopathology studies have utilized the label free microscopy method of Second Harmonic Generation (SHG) to investigate collagen organization in disease onset and progression. Here we explored an alternative label free imaging approach, LC-PolScope that is based on liquid crystal based polarized light imaging. We demonstrated that this more accessible technology has the ability to visualize all fibers of interest and has a good to excellent correlation between SHG and LC-PolScope measurements in fibrillar collagen orientation and alignment. This study supports that LC-PolScope is a viable alternative to SHG for label free collagen organization measurements in thin histology sections.
许多组织病理学研究利用二次谐波产生（SHG）的无标记显微镜方法来研究疾病发生和发展过程中的胶原蛋白组织。在此，我们探索了一种基于液晶偏振光成像的无标记成像替代方法——LC-PolScope。我们证明，这种更容易获得的技术能够可视化所有感兴趣的纤维，并且在纤维状胶原蛋白的取向和排列方面，SHG与LC-PolScope测量之间具有良好到极佳的相关性。这项研究支持LC-PolScope是在薄组织切片中进行无标记胶原蛋白组织测量时SHG的可行替代方法。
