Keikhosravi Adib, Bredfeldt Jeremy S, Sagar Abdul Kader, Eliceiri Kevin W
Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison, USA.
Methods Cell Biol. 2014;123:531-46. doi: 10.1016/B978-0-12-420138-5.00028-8.
The last 30 years has seen great advances in optical microscopy with the introduction of sophisticated fluorescence-based imaging methods such as confocal and multiphoton laser scanning microscopy. There is increasing interest in using these methods to quantitatively examine sources of intrinsic biological contrast including autofluorescent endogenous proteins and light interactions such as second-harmonic generation (SHG) in collagen. In particular, SHG-based microscopy has become a widely used quantitative modality for imaging noncentrosymmetric proteins such as collagen in a diverse range of tissues. Due to the underlying physical origin of the SHG signal, it is highly sensitive to collagen fibril/fiber structure and, importantly, to collagen-associated changes that occur in diseases such as cancer, fibrosis, and connective tissue disorders. An overview of SHG physics background and technologies is presented with a focused review on applications of SHG primarily as applied to cancer.
在过去30年里，随着诸如共聚焦和多光子激光扫描显微镜等复杂的基于荧光的成像方法的引入，光学显微镜取得了巨大进展。人们越来越有兴趣使用这些方法来定量研究内在生物对比度的来源，包括自发荧光的内源性蛋白质以及诸如胶原蛋白中的二次谐波产生（SHG）等光相互作用。特别是，基于SHG的显微镜已成为一种广泛使用的定量成像方式，用于对各种组织中的非中心对称蛋白质（如胶原蛋白）进行成像。由于SHG信号的潜在物理起源，它对胶原纤维/纤维结构高度敏感，重要的是，对癌症、纤维化和结缔组织疾病等疾病中发生的与胶原蛋白相关的变化也高度敏感。本文介绍了SHG的物理背景和技术概述，并重点综述了SHG主要在癌症中的应用。
