Huynh Thao Ngoc, Ren Xiaojun
Department of Chemistry, University of Colorado Denver, Denver, CO, USA.
Bio Protoc. 2017 Jun 20;7(12). doi: 10.21769/BioProtoc.2333.
This protocol describes the production of GST-Cbx7 fusion proteins from , originally developed in the recent publication (Zhen , 2016). The pGEX-6P-1-GST plasmids encoding the Cbx7 variants were transformed into BL21 competent cells. The fusion protein production was induced by isopropyl-beta-D-thiogalactopyranoside and they were purified by Glutathione Sepharose 4B. This protocol can be adapted for the purification of other proteins.
本方案描述了GST-Cbx7融合蛋白的制备方法，该方法最初在最近的出版物（Zhen，2016年）中有所介绍。将编码Cbx7变体的pGEX-6P-1-GST质粒转化到BL21感受态细胞中。融合蛋白的产生通过异丙基-β-D-硫代半乳糖苷诱导，并用谷胱甘肽琼脂糖4B进行纯化。本方案可适用于其他蛋白质的纯化。
