Kurhade Geeta, Nayak B Shivananda, Kurhade Arvind, Unakal Chandrasekhar, Kurhade Krutika
Department of Preclinical Sciences, Faculty of Medical Sciences, The University of West Indies, St. Augustine, Trinidad -
Department of Preclinical Sciences, Faculty of Medical Sciences, The University of West Indies, St. Augustine, Trinidad.
J Sports Med Phys Fitness. 2018 Jul-Aug;58(7-8):1110-1115. doi: 10.23736/S0022-4707.17.07666-6. Epub 2017 Sep 29.
Persistent bouts of extended exercise and heavy training are associated with depressed immune cell function. It has recently been demonstrated that interleukin-6 (IL-6) is produced locally in contracting skeletal muscles and acts on a wide range of tissues. Larger amounts of IL-6 are produced in response to exercise than any other cytokines. Though the majority of existing data obtained following prolonged exercise, it remains to be explained the effect of martial arts training on IL-6 and other immunological parameters and associated changes to the duration of this type of exercise. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, hematopoietic activities, and plays one of the central roles in the regulation of the immune responses. This study aimed to evaluate the effect of martial arts training on IL-6 and other immunological parameters among Trinidadian subjects.
Sixteen healthy, non-smoker individuals who have been martial arts practitioners for the last 5-15 years, aged 25.94±7.6.20 years. Blood samples were collected to determine IL-6 and other immunological parameters at pre-exercise, immediately post exercise (0 hours), 1 hour, 2 hour and 52 hours of post exercise). IL-6 and IL-1 was measured using Human IL-6 and IL-1 β ELISA kit, blood cell count was done using automated blood cell counter and CD4, and CD3 count was performed using the automated immunofluorescence analysis by flow cytometer.
The mean basal IL-6 level was 71.47±4.3 and reduced to 70.1±21.6 immediately after exercise and then increased to 75.70±8.2 after one hour of exercise bout, returning to basal level after two hours and remained so after 52 hours. The CD4 count was decreased as low as 102.2, (much lower than immune-compromised subjects) after the bout of training but returned to normal range within 2 hours of exercise and increased even more after 52 hours. Similar trends have been observed for hematological parameters such as white blood cells, granulocytes and lymphocytes. The white blood cell count, granulocyte count and lymphocyte count increased immediately after exercise and returned to basal level only after 52 hours of exercise.
This study highlights that the martial arts exercise increases key cytokines and other hematological parameters. The magnitude of the martial arts exercise-induced IL-6 response is dependent on intensity and especially duration of the exercise.
持续的长时间运动和高强度训练与免疫细胞功能下降有关。最近有研究表明，白细胞介素-6（IL-6）在收缩的骨骼肌中局部产生，并作用于多种组织。运动后产生的IL-6比其他任何细胞因子都要多。尽管大多数现有数据是在长时间运动后获得的，但武术训练对IL-6和其他免疫参数的影响以及这种运动持续时间的相关变化仍有待解释。IL-1α主要由活化的巨噬细胞产生，也可由中性粒细胞、上皮细胞和内皮细胞产生。它具有代谢、生理、造血活性，在免疫反应调节中发挥核心作用之一。本研究旨在评估武术训练对特立尼达受试者IL-6和其他免疫参数的影响。
16名健康的非吸烟者，在过去5至15年一直练习武术，年龄为25.94±7.620岁。在运动前、运动后即刻（0小时）、运动后1小时、2小时和52小时采集血样，以测定IL-6和其他免疫参数。使用人IL-6和IL-1β ELISA试剂盒测定IL-6和IL-1，使用自动血细胞计数器进行血细胞计数，使用流式细胞仪通过自动免疫荧光分析进行CD4和CD3计数。
基础IL-6平均水平为71.47±4.3，运动后即刻降至70.1±21.6，运动1小时后升至75.70±8.2，2小时后恢复至基础水平，52小时后仍保持该水平。训练后CD4计数低至102.2（远低于免疫功能低下的受试者），但在运动后2小时内恢复到正常范围，52小时后甚至进一步升高。白细胞、粒细胞和淋巴细胞等血液学参数也观察到类似趋势。白细胞计数、粒细胞计数和淋巴细胞计数在运动后即刻升高，仅在运动52小时后恢复至基础水平。
本研究强调武术运动可增加关键细胞因子和其他血液学参数。武术运动诱导的IL-6反应程度取决于运动强度，尤其是运动持续时间。
