Gemba M, Fukuishi N, Nakano S
Division of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Jpn J Pharmacol. 1988 Jan;46(1):90-2. doi: 10.1254/jjp.46.90.
Two days after cisplatin was injected into rats, urinary N-acetyl-beta-D-glucosaminidase (NAG) and gamma-glutamyltranspeptidase (gamma-GTP) activities increased. The urinary excretion of NAG continued to rise until 4 days after the injection of cisplatin, the last day examined. However, the increase in urinary gamma-GTP excretion which lasted for 2 days returned to its control level 4 days after cisplatin injection. The alkaline phosphatase activity in urine was unaffected by cisplatin injections. The antioxidant N-N'-diphenyl-p-phenylenediamine attenuated these increases in enzyme activities caused by cisplatin. The results of this study suggest that monitoring the change in urinary activities of some enzymes is the method of choice for detecting cisplatin nephrotoxicity and that the increase may involve the generation of free radicals by cisplatin.
顺铂注射入大鼠体内两天后，尿中N-乙酰-β-D-氨基葡萄糖苷酶（NAG）和γ-谷氨酰转肽酶（γ-GTP）活性增加。NAG的尿排泄量持续上升，直至顺铂注射后的第4天，即检查的最后一天。然而，持续2天的尿γ-GTP排泄量增加在顺铂注射4天后恢复到对照水平。尿中碱性磷酸酶活性不受顺铂注射的影响。抗氧化剂N-N'-二苯基对苯二胺减弱了顺铂引起的这些酶活性增加。本研究结果表明，监测某些酶的尿活性变化是检测顺铂肾毒性的首选方法，且这种增加可能涉及顺铂产生自由基。
