Köhler Almut, Collymore Chereen, Finger-Baier Karin, Geisler Robert, Kaufmann Larissa, Pounder Kieran C, Schulte-Merker Stefan, Valentim Ana, Varga Zoltan M, Weiss Jürgen, Strähle Uwe
1 Safety and Environment, Karlsruhe Institute of Technology (KIT) , Karlsruhe, Germany .
2 Division of Comparative Medicine, University of Toronto , Toronto, Canada .
Zebrafish. 2017 Dec;14(6):547-551. doi: 10.1089/zeb.2017.1508. Epub 2017 Oct 2.
The increasing importance of zebrafish as a biomedical model organism is reflected by the steadily growing number of publications and laboratories working with this species. Regulatory recommendations for euthanasia as issued in Directive 2010/63/EU are, however, based on experience with fish species used for food production and do not take the small size and specific physiology of zebrafish into account. Consequently, the currently recommended methods of euthanasia in the Directive 2010/63/EU are either not applicable or may interfere with research goals. An international workshop was held in Karlsruhe, Germany, March 9, 2017, to discuss and propose alternative methods for euthanasia of zebrafish. The aim was to identify methods that adequately address the physiology of zebrafish and its use as a biomedical research model, follow the principles of the 3Rs (Replacement, Reduction, and Refinement) in animal experimentation and consider animal welfare during anesthesia and euthanasia. The results of the workshop are summarized here in the form of a white paper.
斑马鱼作为一种生物医学模式生物的重要性日益增加，这体现在使用该物种的出版物和实验室数量稳步增长上。然而，欧盟第2010/63/EU号指令中发布的安乐死监管建议是基于用于食品生产的鱼类物种的经验，并未考虑斑马鱼的小体型和特殊生理学特性。因此，2010/63/EU号指令中目前推荐的安乐死方法要么不适用，要么可能干扰研究目标。2017年3月9日在德国卡尔斯鲁厄举行了一次国际研讨会，以讨论并提出斑马鱼安乐死的替代方法。目的是确定能够充分考虑斑马鱼生理学特性及其作为生物医学研究模型的用途、遵循动物实验中3R原则（替代、减少和优化）并在麻醉和安乐死期间考虑动物福利的方法。研讨会的结果在此以白皮书的形式进行总结。
