Sabzivand Nasim, Nazari Shiva, Shirvani Fariba, Azimi Leila, Salmanzadeh Ahrabi Siavash, Mohammadi Estiri Maedeh
Department of Microbiology, Faculty of Biological Sciences Alzahra University Tehran Iran.
Pediatric Congenital Hematologic Disorders Research Center, Mofid Children Hospital Shahid Beheshti University of Medical Sciences Tehran Iran.
Health Sci Rep. 2024 Jul 31;7(8):e2275. doi: 10.1002/hsr2.2275. eCollection 2024 Aug.
() is the second bacterial cause of nosocomial infections in the general population after . This study surveyed the frequency of cytotoxin-producing strains of and their antibiotic susceptibility profile in a cohort of children admitted to a referral hospital with different malignancies.
The Stool samples of children admitted to the Cancer Chemotherapy Unit of the Mofid Children's Hospital, Tehran, Iran were analyzed using conventional biochemical tests and polymerase chain reaction targeting the gene to identify . The antibiotic susceptibility profile of isolated against commonly prescribed antibiotics used in treating infection at the facility was determined using the Kirby-Bauer disk diffusion technique. Also, the prevalence of genes encoding toxins among was identified by PCR assay.
The Stool samples of 280 participants were taken for the study of which 38 samples [(55.3% (21/38) 42 males and 44.7% (17/38) females)] tested positive for various spp. Out of this, was identified in 2.5% (7/280) stools using cultures and conventional biochemical tests. Also, the stools of 2.9% (8/280) of the participants tested positive for using PCR assay. Using PCR, (2/7) of the isolates tested positive for the and genes and were identified as toxigenic strains.
The prevalence of toxin-producing strains in stool samples of children diagnosed with cancer in Iran is relatively low. Most of the isolates were susceptible to tested antibiotics. Globally, active surveillance of toxigenic strains in patients with different malignancies or immunocompromised patients is recommended in healthcare settings.
（）是普通人群中医院感染的第二大细菌病因，仅次于（）。本研究调查了转诊医院收治的患有不同恶性肿瘤的儿童队列中产细胞毒素菌株的频率及其抗生素敏感性谱。
对伊朗德黑兰莫菲德儿童医院癌症化疗科收治儿童的粪便样本进行分析，采用传统生化试验和针对（）基因的聚合酶链反应来鉴定（）。使用 Kirby-Bauer 纸片扩散技术测定分离出的（）对该机构治疗感染常用抗生素的敏感性谱。此外，通过 PCR 检测确定（）中编码毒素的基因的流行情况。
采集了 280 名参与者的粪便样本进行研究，其中 38 份样本[42 名男性（占 55.3%，21/38）和 44.7%（17/38）女性]检测出各种（）菌呈阳性。其中，通过培养和传统生化试验在 2.5%（7/280）的粪便中鉴定出（）。此外，2.9%（8/280）参与者的粪便样本经 PCR 检测（）呈阳性。通过 PCR 检测，7 株（）分离株中有 2 株（2/7）检测出（）和（）基因呈阳性，被鉴定为产毒素（）菌株。
伊朗诊断为癌症的儿童粪便样本中产毒素（）菌株的流行率相对较低。大多数（）分离株对测试抗生素敏感。在全球范围内，建议在医疗机构中对患有不同恶性肿瘤的患者或免疫功能低下的患者进行产毒素（）菌株的主动监测。
