Lai Zheng-Zong, Yen I-Chuan, Hung Hao-Yuan, Hong Chen-Yang, Lai Chih-Wei, Lee Yen-Mei
Graduate Institute of Medical Science, National Defense Medical Center, Taipei 114, Taiwan.
Department and Graduate Institute of Pharmacology, National Defense Medical Center, Taipei 114, Taiwan.
Pharmaceuticals (Basel). 2024 Jul 26;17(8):988. doi: 10.3390/ph17080988.
Zika virus (ZIKV) and Japanese encephalitis virus (JEV) can cause permanent neurological damage and death, yet no approved drugs exist for these infections. an herb used in traditional Chinese medicine for its antioxidation and antifatigue properties, was studied for its antiviral activity against ZIKV and JEV in vitro. The cytotoxicity of extract (RCE) was evaluated using the CCK-8 reagent. Antiviral effects of RCE were assessed in ZIKV-infected or JEV-infected Vero cells via quantitative reverse transcription polymerase chain reaction (qRT-PCR), Western blotting, fluorescent focus assay (FFA), and immunofluorescence assay (IFA). The cell-free antiviral effects of RCE were evaluated using an inactivation assay. To determine the stage of the viral life cycle affected by RCE, time-of-addition, binding, and entry assays were conducted. Three bioactive constituents of RCE (salidroside, tyrosol, and gallic acid) were tested for antiviral activity. RCE exhibited dose-dependent anti-ZIKV and anti-JEV activities at non-cytotoxic concentrations, which were likely achieved by disrupting viral binding and stability. Gallic acid exhibited antiviral activity against ZIKV and JEV. Our findings indicate that RCE disrupts viral binding and stability, presenting a potential strategy to treat ZIKV and JEV infections.
寨卡病毒（ZIKV）和日本脑炎病毒（JEV）可导致永久性神经损伤和死亡，但目前尚无获批用于治疗这些感染的药物。一种因其抗氧化和抗疲劳特性而用于传统中药的草药，对其在体外抗寨卡病毒和日本脑炎病毒的活性进行了研究。使用CCK-8试剂评估提取物（RCE）的细胞毒性。通过定量逆转录聚合酶链反应（qRT-PCR）、蛋白质印迹法、荧光灶点试验（FFA）和免疫荧光试验（IFA），在寨卡病毒感染或日本脑炎病毒感染的Vero细胞中评估RCE的抗病毒效果。使用灭活试验评估RCE的无细胞抗病毒效果。为确定受RCE影响的病毒生命周期阶段，进行了加样时间、结合和进入试验。对RCE的三种生物活性成分（红景天苷、酪醇和没食子酸）进行了抗病毒活性测试。RCE在非细胞毒性浓度下表现出剂量依赖性的抗寨卡病毒和抗日本脑炎病毒活性，这可能是通过破坏病毒结合和稳定性实现的。没食子酸表现出抗寨卡病毒和抗日本脑炎病毒的活性。我们的研究结果表明，RCE破坏病毒结合和稳定性，为治疗寨卡病毒和日本脑炎病毒感染提供了一种潜在策略。
