通过噬菌体T4复制体理解DNA复制
Understanding DNA replication by the bacteriophage T4 replisome.
作者信息
Benkovic Stephen J, Spiering Michelle M
机构信息
From the Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802
From the Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802.
出版信息
J Biol Chem. 2017 Nov 10;292(45):18434-18442. doi: 10.1074/jbc.R117.811208. Epub 2017 Sep 25.
Abstract
The T4 replisome has provided a unique opportunity to investigate the intricacies of DNA replication. We present a comprehensive review of this system focusing on the following: its 8-protein composition, their individual and synergistic activities, and assembly and into a replisome capable of coordinated leading/lagging strand DNA synthesis. We conclude with a brief comparison with other replisomes with emphasis on how coordinated DNA replication is achieved.
摘要
T4 复制体为研究 DNA 复制的复杂性提供了一个独特的机会。我们对该系统进行了全面综述，重点关注以下方面：其由 8 种蛋白质组成的成分、它们各自的和协同的活性，以及组装成一个能够协调前导链/后随链 DNA 合成的复制体。我们最后简要比较了其他复制体，重点在于如何实现协调的 DNA 复制。
相似文献
1
Understanding DNA replication by the bacteriophage T4 replisome.
J Biol Chem. 2017 Nov 10;292(45):18434-18442. doi: 10.1074/jbc.R117.811208. Epub 2017 Sep 25.
2
Binding Affinities among DNA Helicase-Primase, DNA Polymerase, and Replication Intermediates in the Replisome of Bacteriophage T7.
J Biol Chem. 2016 Jan 15;291(3):1472-80. doi: 10.1074/jbc.M115.698233. Epub 2015 Nov 30.
3
Repetitive lagging strand DNA synthesis by the bacteriophage T4 replisome.
Mol Biosyst. 2008 Nov;4(11):1070-4. doi: 10.1039/b812163j. Epub 2008 Sep 29.
4
Interaction between the T4 helicase loading protein (gp59) and the DNA polymerase (gp43): unlocking of the gp59-gp43-DNA complex to initiate assembly of a fully functional replisome.
Biochemistry. 2005 May 31;44(21):7747-56. doi: 10.1021/bi047296w.
5
The Replication System of Bacteriophage T7.
Enzymes. 2016;39:89-136. doi: 10.1016/bs.enz.2016.02.001. Epub 2016 Mar 28.
6
Dissociative properties of the proteins within the bacteriophage T4 replisome.
J Biol Chem. 2003 Dec 12;278(50):49839-49. doi: 10.1074/jbc.M307405200. Epub 2003 Sep 18.
7
Motors, switches, and contacts in the replisome.
Annu Rev Biochem. 2009;78:205-43. doi: 10.1146/annurev.biochem.78.072407.103248.
8
Coordinated DNA Replication by the Bacteriophage T4 Replisome.
Viruses. 2015 Jun 19;7(6):3186-200. doi: 10.3390/v7062766.
9
Conditional coupling of leading-strand and lagging-strand DNA synthesis at bacteriophage T4 replication forks.
J Biol Chem. 2001 Aug 3;276(31):29559-66. doi: 10.1074/jbc.M101310200. Epub 2001 Jun 4.
10
Dynamic protein interactions in the bacteriophage T4 replisome.
Trends Biochem Sci. 2001 Sep;26(9):566-72. doi: 10.1016/s0968-0004(01)01929-6.
引用本文的文献
1
Expanding the Phage Galaxy: Isolation and Characterization of Five Novel Siphoviruses Ankus, Byblos, DekoNeimoidia, Mandalore, and Naboo.
Phage (New Rochelle). 2024 Sep 16;5(3):153-161. doi: 10.1089/phage.2024.0012. eCollection 2024 Sep.
2
Studies of DNA breathing in exciton-coupled (iCy3) dimer-labeled DNA constructs by polarization-sweep single-molecule fluorescence (PS-SMF) microscopy.
Proc SPIE Int Soc Opt Eng. 2024 Jan-Feb;12863. doi: 10.1117/12.3001962. Epub 2024 Mar 13.
3
Spectroscopic approaches for studies of site-specific DNA base and backbone 'breathing' using exciton-coupled dimer-labeled DNA.
ArXiv. 2024 Mar 27:arXiv:2403.16251v2.
4
Autoinhibition of a clamp-loader ATPase revealed by deep mutagenesis and cryo-EM.
Nat Struct Mol Biol. 2024 Mar;31(3):424-435. doi: 10.1038/s41594-023-01177-3. Epub 2024 Jan 4.
5
Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
Nat Commun. 2023 Jul 20;14(1):4396. doi: 10.1038/s41467-023-40106-2.
6
Structural basis of the T4 bacteriophage primosome assembly and primer synthesis.
bioRxiv. 2023 May 3:2023.05.03.539249. doi: 10.1101/2023.05.03.539249.
7
Studies of Local DNA Backbone Conformation and Conformational Disorder Using Site-Specific Exciton-Coupled Dimer Probe Spectroscopy.
Annu Rev Phys Chem. 2023 Apr 24;74:245-265. doi: 10.1146/annurev-physchem-090419-041204. Epub 2023 Jan 25.
8
Quantitative methods to study helicase, DNA polymerase, and exonuclease coupling during DNA replication.
Methods Enzymol. 2022;672:75-102. doi: 10.1016/bs.mie.2022.03.011. Epub 2022 May 12.
9
Novel Viral DNA Polymerases From Metagenomes Suggest Genomic Sources of Strand-Displacing Biochemical Phenotypes.
Front Microbiol. 2022 Apr 21;13:858366. doi: 10.3389/fmicb.2022.858366. eCollection 2022.
10
Structure of New Binary and Ternary DNA Polymerase Complexes From Bacteriophage RB69.
Front Mol Biosci. 2021 Nov 18;8:704813. doi: 10.3389/fmolb.2021.704813. eCollection 2021.
本文引用的文献
1
Independent and Stochastic Action of DNA Polymerases in the Replisome.
Cell. 2017 Jun 15;169(7):1201-1213.e17. doi: 10.1016/j.cell.2017.05.041.
2
RNA primer-primase complexes serve as the signal for polymerase recycling and Okazaki fragment initiation in T4 phage DNA replication.
Proc Natl Acad Sci U S A. 2017 May 30;114(22):5635-5640. doi: 10.1073/pnas.1620459114. Epub 2017 May 15.
3
The Eukaryotic Replication Machine.
Enzymes. 2016;39:191-229. doi: 10.1016/bs.enz.2016.03.004. Epub 2016 Apr 19.
4
The Replication System of Bacteriophage T7.
Enzymes. 2016;39:89-136. doi: 10.1016/bs.enz.2016.02.001. Epub 2016 Mar 28.
5
The E. coli DNA Replication Fork.
Enzymes. 2016;39:31-88. doi: 10.1016/bs.enz.2016.04.001. Epub 2016 May 13.
6
Single-molecule studies of polymerase dynamics and stoichiometry at the bacteriophage T7 replication machinery.
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4073-8. doi: 10.1073/pnas.1402010111. Epub 2014 Mar 3.
7
Cycling of the E. coli lagging strand polymerase is triggered exclusively by the availability of a new primer at the replication fork.
Nucleic Acids Res. 2014 Feb;42(3):1747-56. doi: 10.1093/nar/gkt1098. Epub 2013 Nov 13.
8
Insights into Okazaki fragment synthesis by the T4 replisome: the fate of lagging-strand holoenzyme components and their influence on Okazaki fragment size.
J Biol Chem. 2013 Jul 19;288(29):20807-20816. doi: 10.1074/jbc.M113.485961. Epub 2013 May 31.
9
A solution to release twisted DNA during chromosome replication by coupled DNA polymerases.
Nature. 2013 Apr 4;496(7443):119-22. doi: 10.1038/nature11988. Epub 2013 Mar 27.
10
Mechanism of strand displacement synthesis by DNA replicative polymerases.
Nucleic Acids Res. 2012 Jul;40(13):6174-86. doi: 10.1093/nar/gks253. Epub 2012 Mar 20.
Zotero 插件