Erdrich Sebastian H, Luthe Tom, Kever Larissa, Badia Roigé Biel, Arsova Borjana, Davoudi Eva, Frunzke Julia
Institute of Bio- and Geosciences, Forschungszentrum Jülich, Jülich, Germany.
Phage (New Rochelle). 2024 Sep 16;5(3):153-161. doi: 10.1089/phage.2024.0012. eCollection 2024 Sep.
Key features of the actinobacterial genus are multicellular, filamentous growth, and production of a broad portfolio of bioactive molecules. These characteristics appear to play an important role in phage-host interactions and are modulated by phages during infection. To accelerate research of such interactions and the investigation of novel immune systems in multicellular bacteria, phage isolation, sequencing, and characterization are needed. This is a prerequisite for establishing systematic collections that appropriately cover phage diversity for comparative analyses.
MATERIAL & METHODS: As part of a public outreach program within the priority program SPP 2330, involving local schools, we describe the isolation and characterization of five novel siphoviruses infecting , , and .
All isolates are virulent members of two existing genera and, additionally, establish a new genus in the family. In addition to an extensive set of tRNAs and proteins involved in phage replication, about 80% of phage genes encode hypothetical proteins, underlining the yet underexplored phage diversity and genomic dark matter still found in bacteriophages infecting actinobacteria.
Taken together, phages Ankus, Byblos, DekoNeimoidia, Mandalore, and Naboo expand the phage diversity and contribute to ongoing research in the field of phage-host interactions.
放线菌属的关键特征是多细胞丝状生长以及产生多种生物活性分子。这些特征似乎在噬菌体 - 宿主相互作用中发挥重要作用，并且在感染过程中会受到噬菌体的调节。为了加速此类相互作用的研究以及多细胞细菌中新型免疫系统的研究，需要进行噬菌体的分离、测序和表征。这是建立能够适当涵盖噬菌体多样性以进行比较分析的系统文库的先决条件。
作为优先计划SPP 2330内公共推广项目的一部分，该项目涉及当地学校，我们描述了五种感染[具体细菌名称]、[具体细菌名称]和[具体细菌名称]的新型长尾噬菌体科病毒的分离和表征。
所有分离株都是两个现有属的烈性成员，此外，还在长尾噬菌体科中建立了一个新属。除了大量参与噬菌体复制的tRNA和蛋白质外，约80%的噬菌体基因编码假定蛋白，这突出了在感染放线菌的噬菌体中仍未充分探索的噬菌体多样性和基因组暗物质。
总之，噬菌体安库斯（Ankus）、比布鲁斯（Byblos）、德科内莫迪亚（DekoNeimoidia）、曼陀罗（Mandalore）和纳布（Naboo）扩展了噬菌体多样性，并为放线菌噬菌体 - 宿主相互作用领域的现有研究做出了贡献。
