埃及地中海贫血症儿童中扭转型肠病毒的系统进化分析。

Phylogenetic Analysis of Torque Teno Virus in Thalassemic Children in Egypt.

机构信息

Department of Medical Microbiology and Immunology, Faculty of Medicine, Minia University, Minia, Egypt.

出版信息

Intervirology. 2017;60(3):102-108. doi: 10.1159/000480507. Epub 2017 Oct 4.

DOI:10.1159/000480507

PMID:28972950

Abstract

OBJECTIVE

Torque teno virus (TTV) is a ubiquitous virus that is commonly associated with blood transfusion. The main aims of this study were to evaluate the incidence of TTV in polytransfused children with thalassemia and to determine for the first time the prevalent TTV genotypes in Egypt.

METHODS

TTV was detected in 2 groups by nested PCR: the first group comprised 200 children with thalassemia, and the second included age- and sex-matched healthy children with no history of blood transfusion.

RESULTS

TTV was detected in 60 and 57%, respectively, of the children with thalassemia and the healthy children. Among the TTV-positive children with thalassemia, 71.6% were HCV positive. No hepatitis B surface antigen was detected in the thalassemic children. Significant elevations of alanine transaminase and aspartate transaminase were found in TTV-positive patients with thalassemia compared to TTV-negative patients. Phylogenetic analysis of sequenced TTV isolates showed close relationships to genotypes 1 and 2.

CONCLUSION

TTV is highly prevalent among children with thalassemia in Egypt, with a relatively high infection rate also detected among healthy children.

摘要

目的

Torque teno 病毒（TTV）是一种普遍存在的病毒，通常与输血有关。本研究的主要目的是评估输血的地中海贫血患儿中 TTV 的发生率，并首次确定埃及流行的 TTV 基因型。

方法

通过巢式 PCR 检测 2 组 TTV：第一组包括 200 名地中海贫血患儿，第二组包括年龄和性别匹配、无输血史的健康儿童。

结果

地中海贫血患儿和健康儿童中 TTV 的检出率分别为 60%和 57%。在 TTV 阳性的地中海贫血患儿中，71.6%的患儿 HCV 阳性。地中海贫血患儿中未检测到乙型肝炎表面抗原。与 TTV 阴性的地中海贫血患儿相比，TTV 阳性的地中海贫血患儿的丙氨酸转氨酶和天冬氨酸转氨酶显著升高。测序的 TTV 分离株的系统发育分析显示与基因型 1 和 2 密切相关。