Carlson J R, Heyworth M F, Owen R L
Cell Immunol. 1986 Jan;97(1):44-50. doi: 10.1016/0008-8749(86)90373-4.
Initial cellular events in the intestinal immune response occur within Peyer's patches and are subject to complex regulation by T cells. The aim of this study was to analyze the response of murine Peyer's patch T, T-helper (Th), and T-suppressor (Ts) cells to Giardia muris infection. Immunocompetent BALB/c mice were infected with G. muris cysts and, at serial times during the infection, Peyer's patch leukocyte suspensions were incubated with fluorescent monoclonal antibodies that identified murine leukocytes, T, Th, or Ts lymphocytes. These suspensions were examined by flow cytometry to quantify each T-cell subset as a percentage of total leukocytes. Total Peyer's patch leukocytes more than doubled in number during the course of G. muris infection and returned to control levels as the infection was cleared. The percentages of Peyer's patch Th and Ts lymphocytes were 34.1 +/- 0.8% (mean +/- SEM) and 6.2 +/- 0.3%, respectively, in the absence of infection, and did not change significantly during Giardia infection. The Th/Ts ratio in Peyer's patches was 5.6 +/- 0.2 in uninfected BALB/c mice and also did not change significantly during clearance of G. muris. We conclude that Peyer's patch leukocytes double in number in response to G. muris infection in immunocompetent mice, G. muris infection does not lead to altered percentages of Peyer's patch T, Th, or Ts lymphocytes, and clearance of G. muris infection is associated with a Peyer's patch Th/Ts ratio of greater than 5.
肠道免疫反应中的初始细胞事件发生在派尔集合淋巴结内，并受到T细胞的复杂调控。本研究的目的是分析小鼠派尔集合淋巴结T细胞、辅助性T细胞（Th）和抑制性T细胞（Ts）对鼠贾第虫感染的反应。将具有免疫活性的BALB/c小鼠感染鼠贾第虫囊肿，并在感染期间的不同时间点，将派尔集合淋巴结白细胞悬液与可识别小鼠白细胞、T细胞、Th或Ts淋巴细胞的荧光单克隆抗体一起孵育。通过流式细胞术检查这些悬液，以量化每个T细胞亚群占总白细胞的百分比。在鼠贾第虫感染过程中，派尔集合淋巴结白细胞总数增加了一倍多，并在感染清除后恢复到对照水平。在未感染的情况下，派尔集合淋巴结Th和Ts淋巴细胞的百分比分别为34.1±0.8%（平均值±标准误）和6.2±0.3%，在贾第虫感染期间没有显著变化。未感染的BALB/c小鼠派尔集合淋巴结中的Th/Ts比值为5.6±0.2，在鼠贾第虫清除过程中也没有显著变化。我们得出结论，在具有免疫活性的小鼠中，派尔集合淋巴结白细胞数量因鼠贾第虫感染而增加一倍，鼠贾第虫感染不会导致派尔集合淋巴结T细胞、Th或Ts淋巴细胞百分比的改变，鼠贾第虫感染的清除与派尔集合淋巴结Th/Ts比值大于5有关。
