Ermak T H, Steger H J, Owen R L, Heyworth M F
Intestinal Immunology Research Center, Veterans Administration Medical Center, San Francisco, CA 94121.
J Histochem Cytochem. 1988 Apr;36(4):417-23. doi: 10.1177/36.4.2964470.
Treatment of mice with anti-L3T4, a monoclonal antibody directed against helper T-cells, impairs clearance of intestinal Giardia muris infection. The present study examined the effect of anti-L3T4 treatment on mouse Peyer's patch cytoarchitecture and on the distribution of T-cell subsets within microenvironments of the follicle. Female BALB/c mice, aged 8 weeks, were given 4-7 weekly injections of either anti-L3T4 (1 mg/wk) or PBS (control group), and Peyer's patches were examined by immunohistochemistry or flow cytometry. In anti-L3T4-treated mice, Peyer's patch follicles (B-cell areas) were about two thirds the size of follicles in controls, and virtually all the size difference occurred in germinal centers. Peyer's patches were depleted of L3T4+ cells, yet the proportion of Thy-1.2+ (all T) cells was not decreased correspondingly, and the distribution of Thy-1.2+ cells in the patches was similar to that in control mice. In anti-L3T4-treated mice, Thy-1.2+ cells consisted of (a) Ly-2+ (cytotoxic/suppressor T) cells, and (b) a population of Thy-1.2+ cells that were neither L3T4+ nor Ly-2+. After treatment, Ly-2+ cells accounted for most of the T-cells in interfollicular areas and were also scattered in follicles, in germinal centers, and below the dome epithelium--in areas where L3T4+ cells predominated in control mice. Thy-1.2+ cells that were L3T4- and Ly-2- were mainly localized below the dome epithelium. These shifts indicate complex interrelationships among different lymphocyte subsets in Peyer's patches.
用抗L3T4（一种针对辅助性T细胞的单克隆抗体）处理小鼠，会损害肠道鼠贾第虫感染的清除。本研究检测了抗L3T4处理对小鼠派尔集合淋巴结细胞结构以及卵泡微环境内T细胞亚群分布的影响。给8周龄的雌性BALB/c小鼠每周注射4 - 7次抗L3T4（1毫克/周）或PBS（对照组），并通过免疫组织化学或流式细胞术检查派尔集合淋巴结。在接受抗L3T4处理的小鼠中，派尔集合淋巴结的卵泡（B细胞区）大小约为对照组卵泡的三分之二，几乎所有大小差异都出现在生发中心。派尔集合淋巴结中L3T4 +细胞减少，但Thy - 1.2 +（所有T）细胞的比例并未相应降低，且这些细胞在淋巴结中的分布与对照小鼠相似。在接受抗L3T4处理的小鼠中，Thy - 1.2 +细胞由（a）Ly - 2 +（细胞毒性/抑制性T）细胞和（b）一群既不是L3T4 +也不是Ly - 2 +的Thy - 1.2 +细胞组成。处理后，Ly - 2 +细胞占滤泡间区大多数T细胞，也散布在卵泡、生发中心以及圆顶上皮下方——这些区域在对照小鼠中L3T4 +细胞占主导。L3T4 -和Ly - 2 -的Thy - 1.2 +细胞主要定位于圆顶上皮下方。这些变化表明派尔集合淋巴结中不同淋巴细胞亚群之间存在复杂的相互关系。
