Jiu Min, Hu Jian, Wang Lun-Ji, Dong Jun-Feng, Song Yue-Qin, Sun Hui-Zhong
College of Food and Bioengineering, Key Laboratory of Microbial Resources Exploitation and Utilization, Henan University of Science and Technology, 471023 Luoyang, China.
Ministry of Agriculture Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Yunnan Provincial Key Lab of Agricultural Biotechnology, Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, 650223 Kunming, China.
J Insect Sci. 2017 May 1;17(3). doi: 10.1093/jisesa/iex048.
Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex, causing significant crop losses in China during the last decade. Although knowledge of cryptic species composition and dynamics within B. tabaci complex is critical for developing sustainable pest management strategies, limited information is available on this pest in the Henan province of China. A systematic survey of the cryptic species composition and distribution of B. tabaci complex in different locations of Henan province was conducted in 2012. The results of RAPD-PCR and the gene for the mitochondrial cytochrome oxidase subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian method indicated there were four known cryptic species MEAM1, MED, Asia II 3, Asia II 9 and a new cryptic species named China 6 in Henan province. In the survey, the invasive cryptic species MED and MEAM1 were found to be predominant with wide spread distribution across the surveyed regions. On the contrary, the indigenous B. tabaci cryptic species including Asia II 3, Asia II 9 and China 6 remained with low prevalence in some surveyed regions. Cryptic species MEAM1 and MED have not completely displaced the native B. tabaci in Henan province. This current study for the first time unifies our knowledge of the diversity and distribution of B. tabaci across Henan province of China.
烟粉虱（Gennadius）（半翅目：粉虱科）是一个隐存种复合体，在过去十年间给中国的农作物造成了重大损失。尽管了解烟粉虱复合体中隐存种的组成和动态对于制定可持续的害虫管理策略至关重要，但关于中国河南省这种害虫的信息却十分有限。2012年，对河南省不同地点烟粉虱复合体的隐存种组成和分布进行了系统调查。基于随机扩增多态性DNA聚合酶链式反应（RAPD-PCR）的结果以及利用贝叶斯方法建立的基于线粒体细胞色素氧化酶亚基1（mtCOI）基因的系统发育关系表明，河南省存在四种已知的隐存种，即中东-小亚细亚1型（MEAM1）、地中海型（MED）、亚洲II 3型、亚洲II 9型以及一个名为中国6型的新隐存种。在调查中，发现入侵性隐存种MED和MEAM1占主导地位，在整个调查区域广泛分布。相反，包括亚洲II 3型、亚洲II 9型和中国6型在内的本地烟粉虱隐存种在一些调查区域的发生率仍然较低。隐存种MEAM1和MED尚未完全取代河南省的本地烟粉虱。这项研究首次统一了我们对中国河南省烟粉虱多样性和分布的认识。
