Masood Mariyam, Amin Imran, Hassan Ishtiaq, Mansoor Shahid, Brown Judith K, Briddon Rob W
Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan.
Pakistan Institute of Engineering and Applied Sciences (PIEAS), Pakistan.
J Econ Entomol. 2017 Dec 5;110(6):2295-2300. doi: 10.1093/jee/tox221.
Bemisia tabaci (Gennadius; Hempitera: Aleyrodidae) is considered to be a cryptic (sibling) species complex, the members of which exhibit morphological invariability while being genetically and behaviorally distinct. Members of the complex are agricultural pests that cause direct damage by feeding on plants, and indirectly by transmitting viruses that cause diseases leading to reduced crop yield and quality. In Pakistan, cotton leaf curl disease, caused by multiple begomovirus species, is the most economically important viral disease of cotton. In the study outlined here, the diversity and geographic distribution of B. tabaci cryptic species was investigated by analyzing a taxonomically informative fragment of the mitochondrial cytochrome c oxidase 1 gene (mtCOI-3'). The mtCOI-3' sequence was determined for 285 adult whiteflies and found to represent six cryptic species, the most numerous being Asia II-1 and Middle East Asia Minor 1 (MEAM-1), the later also referred to as the B-biotype, which was previously thought to be confined to Sindh province but herein, was also found to be present in the Punjab province. The endemic Asia I was restricted to Sindh province, while an individual in the Asia II-8 was identified in Pakistan for the first time. Also for the first time, samples were collected from northwestern Pakistan and Asia II-1 was identified. Results indicate that in Pakistan the overall diversity of B. tabaci cryptic species is high and, based on comparisons with findings from previous studies, the distribution is dynamic.
烟粉虱（Gennadius；半翅目：粉虱科）被认为是一个隐存（姐妹）物种复合体，其成员在形态上具有不变性，但在遗传和行为上却有所不同。该复合体的成员是农业害虫，它们通过取食植物造成直接损害，并通过传播导致疾病的病毒间接造成损害，从而导致作物产量和质量下降。在巴基斯坦，由多种双生病毒引起的棉花卷叶病是棉花最具经济重要性的病毒性病害。在本文所述的研究中，通过分析线粒体细胞色素c氧化酶1基因（mtCOI-3'）的一个分类学信息片段，研究了烟粉虱隐存物种的多样性和地理分布。测定了285只成年粉虱的mtCOI-3'序列，发现其代表六个隐存物种，其中数量最多的是亚洲II-1和中东-小亚细亚1（MEAM-1），后者也被称为B生物型，以前认为仅限于信德省，但在此研究中，也在旁遮普省发现了该生物型。特有种亚洲I仅限于信德省，而亚洲II-8中的一个个体在巴基斯坦首次被鉴定出来。同样也是首次，从巴基斯坦西北部采集了样本，并鉴定出亚洲II-1。结果表明，在巴基斯坦，烟粉虱隐存物种的总体多样性很高，并且根据与以往研究结果的比较，其分布是动态的。
