Souza A L, Lopez-Losano J L, Angelo P C S, Souza-Neto J N, Cordeiro I B, Astolfi-Filho S, Andrade E V
Programa Multi-Institucional de Pós-Graduação em Biotecnologia, , , Brasil.
Fundação de Medicina Tropical do Amazonas, , Brasil.
Genet Mol Res. 2017 Sep 21;16(3):gmr-16-03-gmr.16039642. doi: 10.4238/gmr16039642.
Paullinia cupana Kunth var. sorbilis (Mart.) Ducke, the cultivated guarana plant, is native to the Amazon and has been valued for its medicinal, stimulant and energetic properties for centuries. The seeds are the main commercial product of the plant and the source of high amounts of purine alkaloids (caffeine and theobromine) and polyphenols (flavonoids, catechins, and tannins). Proteins involved in the development and maturation of guarana fruits in its native habitat are interesting issues for proteomics. This study presents the proteomic profile of the seed and pericarp of healthy guarana in different maturation stages. Protein contents were higher in the mature seed compared to other stages due to the accumulation of storage proteins - 11S globulins. Proteins selected for identification by mass spectrometry are mostly related to stress responses and defense and this is not unexpected for fast growing and differentiating reproductive tissues.
栽培的瓜拉那种植物（Paullinia cupana Kunth var. sorbilis (Mart.) Ducke）原产于亚马逊地区，几个世纪以来一直因其药用、刺激和提神特性而受到重视。种子是该植物的主要商业产品，也是大量嘌呤生物碱（咖啡因和可可碱）和多酚（类黄酮、儿茶素和单宁）的来源。参与瓜拉那果实原生栖息地发育和成熟的蛋白质是蛋白质组学研究的有趣课题。本研究展示了不同成熟阶段健康瓜拉那种子和果皮的蛋白质组概况。由于储存蛋白——11S球蛋白的积累，成熟种子中的蛋白质含量高于其他阶段。通过质谱法选择鉴定的蛋白质大多与应激反应和防御有关，这对于快速生长和分化的生殖组织来说并不意外。
