Mercader Josep V, Abad-Somovilla Antonio, Agulló Consuelo, Abad-Fuentes Antonio
Department of Biotechnology, Institute of Agrochemistry and Food Technology (IATA), Spanish National Research Council (CSIC) , Agustí Escardino 7, 46980 Paterna, València, Spain.
Department of Organic Chemistry, Universitat de València , Doctor Moliner 50, 46100 Burjassot, València, Spain.
J Agric Food Chem. 2017 Oct 25;65(42):9333-9341. doi: 10.1021/acs.jafc.7b03199. Epub 2017 Oct 12.
Fluxapyroxad is a new-generation carboxamide fungicide, with residues increasingly being found in food samples. Immunochemical assays have gained acceptance in food quality control as rapid, cost-effective, sensitive, and selective methods for large sample throughput and in situ applications. In the present study, immunoreagents to fluxapyroxad were obtained for the first time, and competitive immunoassays were developed for the sensitive and specific determination of fluxapyroxad residues in food samples. Two carboxyl-functionalized analogues of fluxapyroxad were prepared, and antibodies with IC values in the low nanomolar range were generated from both haptens, though a dissimilar response was observed concerning specificity. A robust direct assay was set up, with a calibration curve exhibiting a limit of detection of 0.05 nM (0.02 μg/L). Limits of quantitation of 5 μg/L were obtained for peach, apple, and grape juices using samples diluted in water. The direct immunoassay was also successfully applied to the determination of fluxapyroxad in grapes from in-field treated grapevines.
氟唑菌酰胺是一种新一代的甲酰胺类杀菌剂，在食品样本中越来越多地检测到其残留。免疫化学分析方法作为一种快速、经济高效、灵敏且具有选择性的方法，适用于大量样本的高通量检测和现场应用，已在食品质量控制中得到广泛认可。在本研究中，首次获得了针对氟唑菌酰胺的免疫试剂，并开发了竞争性免疫分析法，用于灵敏且特异的测定食品样本中的氟唑菌酰胺残留。制备了两种氟唑菌酰胺的羧基官能化类似物，两种半抗原均产生了IC值在低纳摩尔范围内的抗体，不过在特异性方面观察到了不同的反应。建立了一种稳健的直接分析法，校准曲线的检测限为0.05 nM（0.02 μg/L）。使用用水稀释的样本，桃汁、苹果汁和葡萄汁的定量限为5 μg/L。直接免疫分析法还成功应用于测定田间处理过的葡萄藤上葡萄中的氟唑菌酰胺。
