Institute of Food and Drug Inspection, Zhoukou Normal University, Zhoukou, 466001, P.R. China.
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, P.R. China.
Sci Rep. 2020 Feb 24;10(1):3270. doi: 10.1038/s41598-020-59112-1.
An ultrasensitive indirect competitive enzyme-linked immunosorbent assay (ic ELISA) using monoclonal antibodies (mAbs) was developed for the specific detection of diethylstilbestrol (DES) residues. To establish an ELISA based on mAbs, hapten diethylstilbestrol mono-carboxypropyl-ether (DES-MCPE) was chemically synthetized and then conjugated to bovine serum albumin (BSA) for immunization in mice. This ic ELISA was further optimized for DES determination. The sensitivity of the ic ELISA was found to be 0.49 μg/kg and the limit of detection was 0.075 μg/kg. DES residues in salmon meat and pork were tested with the recovery range from 74.0 to 85.2% and the coefficient of variation (CV) was less than 10%. Parallel analysis of DES samples from salmon meat showed comparable results from the ic ELISA with high-performance liquid chromatography. The ic ELISA provides a useful screening method for the quantitative detection of DES residues in animal-derived food.
建立了一种基于单克隆抗体（mAb）的间接竞争酶联免疫吸附测定法（icELISA），用于特异性检测己烯雌酚（DES）残留。为了建立基于 mAb 的 ELISA，化学合成了半抗原己烯雌酚单羧丙基醚（DES-MCPE），并将其与牛血清白蛋白（BSA）偶联，用于免疫小鼠。进一步优化了 icELISA 用于 DES 的测定。icELISA 的灵敏度为 0.49μg/kg，检测限为 0.075μg/kg。用该方法检测三文鱼和猪肉中的 DES 残留，回收率在 74.0%至 85.2%之间，变异系数（CV）小于 10%。用 icELISA 平行分析三文鱼中 DES 样品的结果与高效液相色谱法的结果相当。icELISA 为动物源性食品中 DES 残留的定量检测提供了一种有用的筛选方法。
