使用蛋白酶K和苯酚从哺乳动物血液中分离高分子量DNA
Isolation of High-Molecular-Weight DNA from Mammalian Blood Using Proteinase K and Phenol.
作者信息
Green Michael R, Sambrook Joseph
出版信息
Cold Spring Harb Protoc. 2017 Oct 3;2017(10):pdb.prot093492. doi: 10.1101/pdb.prot093492.
PMID:28974655
Abstract
This procedure is the method of choice for purification of genomic DNA from mammalian blood when large amounts of DNA are required, for example, for Southern blotting. The usual yield of DNA from buffy coat lymphocytes isolated from 20 mL of normal blood is ∼250 µg.
摘要
当需要大量DNA（例如用于Southern印迹法）时，此方法是从哺乳动物血液中纯化基因组DNA的首选方法。从20 mL正常血液中分离出的血沉棕黄层淋巴细胞通常可获得约250 μg的DNA产量。
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