使用蛋白酶K和苯酚从哺乳动物细胞悬浮培养物中分离高分子量DNA
Isolation of High-Molecular-Weight DNA from Suspension Cultures of Mammalian Cells Using Proteinase K and Phenol.
作者信息
Green Michael R, Sambrook Joseph
出版信息
Cold Spring Harb Protoc. 2018 Apr 2;2018(4):2018/4/pdb.prot093476. doi: 10.1101/pdb.prot093476.
PMID:29610360
Abstract
This procedure is the method of choice for purification of mammalian genomic DNA from suspension cultures when large amounts of DNA are required, for example, for Southern blotting. Approximately 200 µg of mammalian DNA, 100-150 kb in length, is obtained from 5 × 10 cultured aneuploid cells (e.g., HeLa cells).
摘要
当需要大量DNA（例如用于Southern印迹法）时，此方法是从悬浮培养物中纯化哺乳动物基因组DNA的首选方法。从5×10个培养的非整倍体细胞（如HeLa细胞）中可获得约200μg长度为100 - 150kb的哺乳动物DNA。
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