从革兰氏阴性菌中分离DNA。
Isolating DNA from Gram-Negative Bacteria.
作者信息
Green Michael R, Sambrook Joseph
出版信息
Cold Spring Harb Protoc. 2017 Jan 3;2017(1):2017/1/pdb.prot093369. doi: 10.1101/pdb.prot093369.
PMID:28049781
Abstract
The isolation of DNA from bacteria, described in this protocol, relies upon the use of sodium dodecyl sulfate and proteinase K to lyse the cells. High-molecular-weight DNA is then sheared (to reduce its viscosity and make it more manageable), extracted with phenol:chloroform, and precipitated with isopropanol. DNA isolated according to this procedure ranges from 30 to 80 kb in length.
摘要
本实验方案中所描述的从细菌中分离DNA的方法，依赖于使用十二烷基硫酸钠和蛋白酶K来裂解细胞。然后将高分子量的DNA进行剪切（以降低其粘度并使其更易于处理），用酚：氯仿进行提取，并用异丙醇沉淀。按照此程序分离得到的DNA长度在30至80 kb之间。
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