Arabsolghar Rita, Saberzadeh Jamileh, Khodaei Forouzan, Borojeni Rozhin Abbasi, Khorsand Marjan, Rashedinia Marzieh
Department of Laboratory Sciences and Diagnostic Laboratory Sciences & Technology Research Center, Paramedical School, Shiraz University of Medical Sciences, Shiraz, I.R. Iran.
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, I.R. Iran.
Res Pharm Sci. 2017 Oct;12(5):391-400. doi: 10.4103/1735-5362.213984.
Sodium benzoate (SB) is one of the food additives and preservatives that prevent the growth of fungi and bacteria. SB has been shown to improve the symptoms of neurodegenerative disease such as Alzheimer's disease. The aim of this study was to evaluate the effect of SB on the cell survival and cellular antioxidant indices after exposure to aluminum maltolate (Almal) in PC12 cell line as a model of neurotoxicity. The cells exposed to different concentrations of SB (0.125 to 3 mg/mL) in the presence of Almal (500 µM) and cell viability, the level of reactive oxygen species (ROS), glutathione content and catalase activity were measured. The results showed that low concentrations of SB caused an increase in the cell survival, but cell viability was reduced in high concentrations. SB could neither prevent the level of ROS production nor change glutathione content. SB (0.5 mg/mL) significantly increased the catalase enzyme activity as compared to the Almal. This study suggested that SB did not completely protect the cell to aluminum-induced free radicals toxicity. Possibly SB improves the symptoms of neurodegenerative disease by other mechanisms.
苯甲酸钠（SB）是一种食品添加剂和防腐剂，可防止真菌和细菌生长。已证明SB可改善神经退行性疾病如阿尔茨海默病的症状。本研究的目的是在作为神经毒性模型的PC12细胞系中，评估SB对暴露于苹果酸铝（Almal）后细胞存活和细胞抗氧化指标的影响。在存在Almal（500 μM）的情况下，将细胞暴露于不同浓度的SB（0.125至3 mg/mL），并测量细胞活力、活性氧（ROS）水平、谷胱甘肽含量和过氧化氢酶活性。结果表明，低浓度的SB可导致细胞存活率增加，但高浓度时细胞活力降低。SB既不能阻止ROS的产生水平，也不能改变谷胱甘肽含量。与Almal相比，SB（0.5 mg/mL）显著提高了过氧化氢酶活性。本研究表明，SB不能完全保护细胞免受铝诱导的自由基毒性。SB可能通过其他机制改善神经退行性疾病的症状。
