长链非编码 RNA MEG3 通过 p53 信号通路抑制胃癌的增殖和转移。
lncRNA MEG3 inhibit proliferation and metastasis of gastric cancer via p53 signaling pathway.
机构信息
Department of Pathology, Jinzhou Medical University, Jinzhou, Liaoning Province, China.
出版信息
Eur Rev Med Pharmacol Sci. 2017 Oct;21(17):3850-3856.
OBJECTIVE
lncRNA MEG3 has been reported as a tumor suppressor gene in many different kinds of cancer, but its role in gastric cancer has not been fully understood. Then, we would like to explore its mechanism in gastric cancer.
PATIENTS AND METHODS
We first used qRT-PCR to detect the expression of lncRNA MEG3, then CCK8 and wound healing assay were used to detect the effect of lncRNA MEG3 on gastric cancer cells. Western blot assay was used to measure the expression of p53 when lncRNA MEG3 was overexpressed.
RESULTS
lncRNA MEG3 was highly expressed in the adjacent tissue, compared to the one in gastric cancer tissue. What's more, we also found that overexpression of lncRNA MEG3 could decrease the proliferation and metastasis of gastric cancer cells. Finally, overexpression of lncRNA MEG3 could also increase the expression of p53.
CONCLUSIONS
lncRNA MEG3 could inhibit the proliferation and metastasis of gastric cancer.
目的
长链非编码 RNA MEG3 已被报道在许多不同类型的癌症中作为肿瘤抑制基因，但它在胃癌中的作用尚未完全阐明。因此，我们希望探索其在胃癌中的作用机制。
患者与方法
我们首先使用 qRT-PCR 检测长链非编码 RNA MEG3 的表达，然后使用 CCK8 和划痕愈合实验检测长链非编码 RNA MEG3 对胃癌细胞的影响。使用 Western blot 检测当长链非编码 RNA MEG3 过表达时 p53 的表达。
结果
与胃癌组织相比，长链非编码 RNA MEG3 在相邻组织中高表达。此外，我们还发现过表达长链非编码 RNA MEG3 可降低胃癌细胞的增殖和转移。最后，过表达长链非编码 RNA MEG3 还可以增加 p53 的表达。
结论
长链非编码 RNA MEG3 可以抑制胃癌的增殖和转移。
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