Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Stomatology Service of Hospital Odilon Behrens, Belo Horizonte, Brazil.
J Oral Pathol Med. 2018 Jan;47(1):91-95. doi: 10.1111/jop.12645. Epub 2017 Oct 26.
The oral lichen planus is a chronic inflammatory disease. Although its aetiology is not well understood, the role of T lymphocytes in its inflammatory events is recognised. Identifying the epigenetic mechanisms involved in the pathogenesis of this immune-mediated condition is fundamental for understanding the inflammatory reaction that occurs in the disease. The purpose of this work was to evaluate the methylation pattern of 21 immune response-related genes in the different clinical forms of oral lichen planus.
A cross-sectional study was performed to analyse the DNA methylation patterns in three distinct groups of oral lichen planus: (i) reticular/plaque lesions; (ii) erosive lesions; (iii) normal oral mucosa (control group). After DNA extraction from biopsies, the samples were submitted to digestions by methylation-sensitive and methylation-dependent enzymes and double digestion. The relative percentage of methylated DNA for each gene was provided using real-time polymerase chain reaction arrays.
Hypermethylation of the STAT5A gene was observed only in the control group (59.0%). A higher hypermethylation of the ELANE gene was found in reticular/plaque lesions (72.1%) compared to the erosive lesions (50.0%).
Our results show variations in the methylation profile of immune response-related genes, according to the clinical type of oral lichen planus after comparing with the normal oral mucosa. Further studies are necessary to validate these findings using gene expression analysis.
口腔扁平苔藓是一种慢性炎症性疾病。尽管其病因尚未完全明确,但人们已经认识到 T 淋巴细胞在其炎症事件中的作用。确定参与这种免疫介导的疾病发病机制的表观遗传机制对于理解疾病中发生的炎症反应至关重要。本研究旨在评估 21 种与免疫反应相关基因在口腔扁平苔藓不同临床形式中的甲基化模式。
进行了一项横断面研究,以分析三组不同的口腔扁平苔藓的 DNA 甲基化模式:(i)网状/斑块病变;(ii)糜烂性病变;(iii)正常口腔黏膜(对照组)。从活检组织中提取 DNA 后,将样本进行甲基化敏感和甲基化依赖性酶消化以及双消化。使用实时聚合酶链反应阵列提供每个基因的甲基化 DNA 相对百分比。
仅在对照组中观察到 STAT5A 基因的高甲基化(59.0%)。与糜烂性病变(50.0%)相比,网状/斑块病变中 ELANE 基因的高甲基化程度更高(72.1%)。
与正常口腔黏膜相比,我们的结果表明,根据口腔扁平苔藓的临床类型,与免疫反应相关的基因的甲基化谱存在差异。需要进一步的研究来使用基因表达分析验证这些发现。