辅助性T细胞17、初始辅助性T细胞及辅助性T细胞2在糜烂型和网状口腔扁平苔藓中的潜在致病作用

Potential pathogenetic role of Th17, Th0, and Th2 cells in erosive and reticular oral lichen planus.

作者信息

Piccinni M-P, Lombardelli L, Logiodice F, Tesi D, Kullolli O, Biagiotti R, Giudizi Mg, Romagnani S, Maggi E, Ficarra G

机构信息

Department of Experimental and Clinical Medicine, Center of Excellence DENOTHE, University of Florence, Florence, Italy.

出版信息

Oral Dis. 2014 Mar;20(2):212-8. doi: 10.1111/odi.12094. Epub 2013 Apr 5.

DOI:10.1111/odi.12094

Abstract

OBJECTIVES

The role of Th17 cells and associated cytokines was investigated in oral lichen planus.

MATERIAL AND METHODS

14 consecutive patients with oral lichen planus were investigated. For biological studies, tissues were taken from reticular or erosive lesions and from normal oral mucosa (controls) of the same patient. mRNA expression for IL-17F, IL-17A, MCP-1, IL-13, IL-2, IL-10, IL-1β, RANTES, IL-4, IL-12B, IL-8, IFN-γ, TNF-α, IL-1α, IL-18, TGF-β1, IL-23R, IL-7, IL-15, IL-6, MIG, IP-10, LTB, VEGF, IL-5, IL-27, IL-23A, GAPDH, PPIB, Foxp3, GATA3, and RORC was measured using the QuantiGene 2.0.

RESULTS

Results showed that Th17-type and Th0-type molecules' mRNAs, when compared with results obtained from tissue controls, were increased in biopsies of erosive lesions, whereas Th2-type molecules' mRNAs were increased in reticular lesions. When the CD4+ T-cell clones, derived from oral lichen planus tissues and tissue controls, were analyzed, a higher prevalence of Th17 (confirmed by an increased CD161 expression) and Th0 CD4+ T clones was found in erosive lesions, whereas a prevalence of Th2 clones was observed in reticular lesions.

CONCLUSIONS

Our data suggest that Th17, Th0, and Th2 cells, respectively, may have a role in the pathogenesis of erosive and reticular oral lichen planus.

摘要

目的

研究Th17细胞及相关细胞因子在口腔扁平苔藓中的作用。

材料与方法

对14例连续的口腔扁平苔藓患者进行研究。为进行生物学研究，取自同一患者的网状或糜烂性病变组织以及正常口腔黏膜（对照）。使用QuantiGene 2.0检测IL-17F、IL-17A、MCP-1、IL-13、IL-2、IL-10、IL-1β、RANTES、IL-4、IL-12B、IL-8、IFN-γ、TNF-α、IL-1α、IL-18、TGF-β1、IL-23R、IL-7、IL-15、IL-6、MIG、IP-10、LTB、VEGF、IL-5、IL-27、IL-23A、GAPDH、PPIB、Foxp3、GATA3和RORC的mRNA表达。

结果

结果显示，与组织对照结果相比，糜烂性病变活检中Th17型和Th0型分子的mRNA增加，而网状病变中Th2型分子的mRNA增加。当分析源自口腔扁平苔藓组织和组织对照的CD4+T细胞克隆时，糜烂性病变中发现Th17（通过CD161表达增加证实）和Th0 CD4+T克隆的患病率更高，而网状病变中观察到Th2克隆的患病率。

结论

我们的数据表明，Th17、Th0和Th2细胞可能分别在糜烂性和网状口腔扁平苔藓的发病机制中起作用。