Denadai Amanda Silveira, Aydos Ricardo Dutra, Silva Iandara Schettert, Olmedo Larissa, de Senna Cardoso Bruno Mendonça, da Silva Baldomero Antonio Kato, de Carvalho Paulo de Tarso Camillo
Federal University of Mato Grosso do Sul (UFMS), Post Graduate Program in Health and Development of Midwest Region, Campo Grande/MS, Brazil.
Federal University of Piauí (UFPI), Department of Health Sciences, Parnaíba/PI, Brazil.
J Exp Ther Oncol. 2017 Sep;11(2):85-89.
Laser therapy influences oxidative stress parameters such as the activity of antioxidant enzymes and the production of reactive oxygen species.
To analyze the effects of low-level laser therapy on oxidative stress in diabetics rats with skin wounds.
Thirty-six animals were divided into 4 groups: NDNI: non-diabetic rats with cutaneous wounds that not received laser therapy; NDI: non-diabetic rats with cutaneous wounds that received laser therapy; DNI: diabetic rats with skin wounds who did not undergo laser therapy; DI: rats with diabetes insipidus and cutaneous wounds and received laser therapy. The animals were treated with LLLT (660 nm, 100 mW, 6 J/cm, spot size 0.028 cm). On the day of killing the animals, tissue-wrapped cutaneous wounds were collected and immediately frozen, centrifuged, and stored to analyze malondialdehyde (MDA) levels.
Significant difference was observed within the groups of MDA levels (ANOVA, p = 0.0001). Tukey's post-hoc test showed significantly lower values of MDA in irradiated tissues, both in diabetic and non-diabetic rats. ANOVA of the diabetic group revealed a significant difference (p < 0.01) when all groups, except NDI and DI, were compared.
LLLT was effective in decreasing MDA levels in acute surgical wounds in diabetic rats.
激光疗法会影响氧化应激参数，如抗氧化酶的活性和活性氧的产生。
分析低强度激光疗法对糖尿病皮肤创伤大鼠氧化应激的影响。
将36只动物分为4组：NDNI：有皮肤创伤但未接受激光治疗的非糖尿病大鼠；NDI：有皮肤创伤且接受激光治疗的非糖尿病大鼠；DNI：有皮肤创伤但未接受激光治疗的糖尿病大鼠；DI：有尿崩症和皮肤创伤且接受激光治疗的大鼠。对动物进行低强度激光疗法治疗（660纳米，100毫瓦，6焦/平方厘米，光斑大小0.028平方厘米）。在处死动物当天，收集包裹有组织的皮肤创伤样本，立即冷冻、离心并储存，以分析丙二醛（MDA）水平。
在MDA水平组间观察到显著差异（方差分析，p = 0.0001）。Tukey事后检验显示，在糖尿病和非糖尿病大鼠中，接受照射组织的MDA值均显著较低。对糖尿病组进行方差分析发现，当比较除NDI和DI之外的所有组时，存在显著差异（p < 0.01）。
低强度激光疗法可有效降低糖尿病大鼠急性手术创伤中的MDA水平。
