Macedo Cesar Augusto Barbosa de, Macedo Madlaine Frigo Silveira Barbosa de, Miura Ana Carolina, Taroda Alessandra, Cardim Sergio Tosi, Innes Elisabeth Ann, Katzer Frank, Cantón German Jose, Chianini Francesca, Headley Selwyn Arlington, Garcia João Luis
Companhia Integrada de Desenvolvimento Agrícola de Santa Catarina - CIDASC, Presidente Getúlio, SC, Brasil.
Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina - UEL, Londrina, PR, Brasil.
Rev Bras Parasitol Vet. 2017 Jul-Sep;26(3):292-298. doi: 10.1590/S1984-29612017051.
The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.
本研究的目的是通过酶联免疫吸附测定（ELISA）、免疫组织化学（IHC）和聚合酶链反应（PCR），调查巴西南部圣卡塔琳娜州奶牛流产中与犬新孢子虫相关的情况。收集了流产奶牛的血液以及其胎儿的胸腔积液和组织样本（脑、心脏、肝脏和肺）用于血清学检测；还进行了PCR、组织病理学和免疫组织化学（IHC）评估。41头奶牛中有21头（51.2%），30个胎儿中有8个（26.7%）犬新孢子虫ELISA（HerdCheck，IDEXX）检测呈阳性。年龄大于36个月的奶牛血清呈阳性的风险高于年轻动物。PCR和IHC显示，分别有38.8%（14/36）和25.0%（9/36）的胎儿犬新孢子虫检测呈阳性。血清阳性的奶牛所产胎儿通过胸腔积液、PCR或IHC检测呈阳性的频率更高。总之，本研究观察到巴西南部奶牛流产中犬新孢子虫的感染频率较高，36个月以上的奶牛中犬新孢子虫的患病率更高。此外，血清学、PCR和IHC应一起使用，以更好地诊断牛的新孢子虫病。
